Part - 3: Select the Right Purification Approach for Your Process
Webinar Series: Your mRNA Process, Simplified
With mRNA vaccines serving as a major component in the fight against SARS-CoV-2 and the continued development of therapies against various indications, the need to produce mRNA has never been greater. In this three-part webinar series, experts from Sartorius demonstrate how improved processes, analytics, and tools are key in meeting that demand.
In this final webinar of the series, Sartorius purification experts demonstrate the flexibility that can be achieved with an mRNA purification toolbox featuring monolithic chromatography. As a new and rapidly developing field, one of the challenges for mRNA therapeutics and vaccines is the lack of standardization. With multiple production platforms, processes vary – as do the impurities that may be introduced. This impedes the development of a single purification platform.
- Show how to break down bottlenecks with a monolithic chromatography toolbox that offers purification solutions across multiple mRNA production platforms
- Demonstrate how to implement different capture chromatography steps for purification of mRNA from IVT reactions
- Share a scalable purification solution for commercial manufacturing of mRNA therapeutics
- Become familiar with monolithic chromatography solutions designed to overcome challenges in mRNA purification
- Learn how to implement affinity and non-affinity capture of mRNA from an IVT reaction mixture using CIMmultus® monoliths
Meet Our pDNA & mRNA Expert
Tomas Kostelec
Product Manager mRNA | pDNA
Tomas Kostelec has completed his Master’s in organic chemistry in Chemistry at University College London, with a focus on multimodal contrast agents for medical imaging. He joined BIA Separations, now part of Sartorius in 2016 as analytical scientist to support contract work and development activities for AAV, influenza, and other viruses. He is currently Product Manager for mRNA at Sartorius, his area of interest includes purification and analytics of mRNA.
Part – 1: Increase pDNA Purity and Yields
In part one you will learn how to achieve higher yields in the plasmid DNA by ensuring the quality and purity of the raw materials and taking other steps to optimize the production process.
What Will You Learn
- Review strategies to minimize introduction of product-related contaminants in the IVT reaction
- Explore ready-to-use solutions for scalable chromatographic purification of plasmid DNA
- See real-world impact of the HiP2 Plasmid Process Pack for purification of linearized pDNA
Part - 2: Break Down the mRNA Bottleneck with Rapid Analytical Strategies
In part two of the 'Your mRNA process, Simplified' webinar series, we continue to explore how improved processes, analytics, and tools can help meet the unprecedented demand for mRNA.
What You Will Learn
- Discover how analytical HPLC with CIMac columns can improve in-process control and quality control of the mRNA production process
- Understand the value of rapid analytics during process optimization, for process monitoring and final quality control
- Review the real-world results of analytical strategies implemented in mRNA production
Part - 3: Select the Right Purification Approach for Your Process
In this final webinar of the series, Sartorius purification experts demonstrate the flexibility that can be achieved with an mRNA purification toolbox featuring monolithic chromatography.
What You Will Learn
- Discover a scalable purification solution for commercial manufacturing of mRNA therapeutics
- Become familiar with monolithic chromatography solutions designed to overcome challenges in mRNA purification
- Learn how to implement affinity and non-affinity capture of mRNA from an IVT reaction mixture using CIMmultus® monoliths