Biophysical Characterization of the Binding of SARS-CoV-2 to the ACE2 Protein Using the Octet® R4 Bio-Layer Interferometry Platform Application Note
Molecular level studies are routinely used to understand the determinants of infectivity for viruses, such as the binding interaction between SARS-CoV-2 to cells of respiratory mucosa through the angiotensin converting enzyme 2 (ACE2) receptors in the human body.
In this application note, we use the Octet® Bio-Layer Interferometry (BLI) platform to characterize the binding of SARS-CoV-2 to the various molecules engaged during an infection (ACE2 protein, spike protein-S, receptor binding domain and the trimeric SARS-CoV-2 S protein). See how this advanced system allows you to quickly evaluate the thermodynamics at play during molecular interactions.
Document Type: Application Note
Page Count: 8
Read Time: 15 minutes
Last Updated: December 1, 2022
Authors: Nilshad Salim PhD, Bob Dass PhD, and David Apiyo PhD
Target Audience: Immunologists, Viral Disease Researchers
Applications Covered: Biology, Pathogenesis, Immunomodulation