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Cell Culture Protein Analysis White Papers, Infographics, and Posters

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Sartorius offers a broad collection of materials to enhance user knowledge and confidence when it comes to cell culture techniques. Gain insight on ways to reduce the risk of contamination and control environmental factors to improve the health of your culture. Sartorius offers a wide range of materials to enhance your cell culture and cell assay knowledge and confidence with up-to-date cell culture solutions and analysis technologies. Sartorius white papers, posters, webinars, infographics, application notes, and much more are all at your disposal. Gain the insight needed to reduce contamination risk and other environmental factors. We have everything you need to ensure healthy cell culture and hypothesis testing.

Live-Cell Analysis of Cell Subsets and Heterogeneity

Considerable heterogeneity exists in the properties and activity of individual cells, even in the simplest cell system. This arises from fundamental differences in the basic cell types present (e.g. tumor cells and fibroblasts), genetic or epigenetic variations, the stage of cell cycle or differentiation, and the impact of each cells’ unique and local dynamic microenvironment. Cellular plasticity and age-related changes add a further, long-term temporal complexity.

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Cell Culture Quality Control

The IncuCyte® Live-Cell Analysis System is an essential cell culture quality control tool. The IncuCyte is an automated microscope that provides a label-free, non-invasive method for monitoring cells at regular time intervals right inside your cell culture incubator, and thus provides quantitative, objective, non-invasive, and kinetic data on your living cells in their native environment. Your resulting data consist of objective, image-based growth metrics, giving you key information you need to control adverse variables that alter the growth and function of your cells. 

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Benchmarking of the Microsart® AMP Mycoplasma kit

The traditional growth-based method of mycoplasma detection requires a cultivation time of at least 28 days before a contamination can be ruled out with certainty. In comparison, Nucleic Acid Amplification Techniques (NAT) reduce time to results to just hours. 

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Lab-Scale Clarification of Mammalian Suspension Cultures Using Sartoclear Dynamics® Lab V Kits

This study evaluates a novel method for lab-scale clarification of mammalian cell culture supernatants. The filtration step is conducted prior to purification of monoclonal antibodies with protein A affinity chromatography. In a conventional clarification process, cells are removed by a time-consuming centrifugation step followed by filtration. We show that the Sartoclear Dynamics® Lab V Kit is not only a significant time-saving method compared with the conventional clarification process. It also yields an improved clearance rate that we observed in combination with high flow rate filtration. For clarification of large-volume cell cultures, Sartoclear Dynamics® Lab V Kits are an attractive addition to standard lab instrumentation to increase productivity and throughput prior to protein purification.

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Pipette Essentials - Make Your Choice & How to Select a Mechanical Pipette

Your choice of pipette and pipette tip can make a difference when pipetting cells from the seed and working stocks of your established cell lines. Download our checklist of Pipette Essentials & How to Select a Mechanical Pipette Guide when determining the right pipette for your lab needs. 

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Preventing Cell Death

The presence of endotoxins can lead to cell death in mammalian cell cultures. For this reason, ultrapure media, i.e., ultrapure water, with levels proven to be below the limits must be used to manufacture biopharmaceuticals or to propagate cell lines or cell cultures. This study demonstrates that the ultrapure water produced by the Arium® Pro VF water purification system exhibits an endotoxin content that is far below the prescribed limits and that can be used for applications of these categories.

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Application Note: Sartorius Ultrafiltration Products in the Preparation of Biological and Medical Nanocarriers – a Short Review

This short review provides examples of recent literature dealing with the preparation of Nanoparticles. Particular focus is laid on the concentration and purification steps which were performed via ultrafiltration with Sartorius Vivaspin® or Vivaflow® devices with different pore sizes (MWCO).

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Top Ten Tips To Avoid Contamination In Pipetting

Do you know how to avoid contamination in pipetting? One of the most essential—but overlooked—methods of cell culture contamination prevention is proper aseptic pipetting technique, including choosing and using the appropriate pipettes and pipette tips. Download this poster and get 10 tips for contamination-free pipetting!

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Water is Life: Ultrapure Water in Cell Cultivation

Water is a major component of all cell culture media and is therefore needed to prepare media, buffers, and additives, and as well as to serve many ancillary functions, such as heating, cooling, cleaning and rinsing. Thus, water quality plays an important role in the outcome of cell culture experiments. 

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Minimize Cell Culture Contamination

Batch failure due to cell culture contamination is a serious consideration for biomanufacturing. A recent BioPlan Associates Survey found that on average a failure occurs every 9.4 months and costs companies $1 to $2 million. The leading causes of batch failure were contamination and operator error. It is logical that anytime the cell culture system is open, for instance when opening flasks during cell expansion, there is an opportunity for contamination.

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Infographic: Critical Challenges in Cell-Based Analysis — A Roadmap

Learn the advantages of live-cell imaging and how it will allow you to circumvent the most critical challenges in cell-based assays. 

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Application Note: Minimize the Risk of Mycoplasma Contamination in Your Cell Cultures

Preventing mycoplasma contamination in the cell culture lab can be a challenge. However, you can significantly reduce the risk of mycoplasma contamination and spread in your cell culture lab with good cell culture practices, by selecting easy-to-clean pipettes and sterility promoting consumables, and by regularly testing.

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