Achieving Scalable, High-Yield Plasmid DNA Purification Processes

Discover effective strategies to tackle bottlenecks in plasmid (p)DNA downstream processing. Explore real-world process data to optimize each step—from lysis to sterile filtration—ensuring maximum yield, purity, and regulatory compliance.

Case studies showcase a fully integrated, end-to-end pDNA purification process, achieving over 98% integrity and 64% recovery of supercoiled pDNA, more than 95% sc pDNA in lysate with automated in-line lysis, over 90% recovery rates for AEX and HIC steps, and less than 4% loss in final UF/DF and sterile filtration.

Learn how to:

Tackle scale-up challenges in pDNA production, including effective control over lysis and chromatographic separation at large scale.
Maximize recovery and consistency at gram-scale while adhering to regulatory standards.
Implement continuous plasmid purification to maintain pDNA quality.
Utilize at-line analytics to monitor and optimize purification in real time.

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