Purification of Coagulation Factor IX from Cryopoor Plasma Using CIMmultus^® Chromatography 

This poster describes a two-step purification process for coagulation factor IX (FIX) from undiluted cryoprecipitate plasma using CIMmultus^® DEAE (capture) and CIMmultus^® SO4 (polishing) monolithic columns.  

The CIMmultus^® chromatography enables the rapid processing of large plasma volumes and direct loading without prior dilution, which addresses the limitations of conventional diffusive-based resins. 

The CIMmultus^® DEAE step achieved 49% FIX recovery, while the CIMmultus^® SO4 polishing step yielded 93% recovery and a final FIX specific activity of 455 IU/mg protein. Co-purified vitamin K–dependent coagulation factors (FII, FVII, and FX) were reduced to below the limit of quantification in the main CIMmultus^® SO4 elution fraction.