Plenty of Lenti - A multi-angle approach towards method optimization of lentivirus production

Lentiviral vectors (LVs) are increasingly used for cell therapy applications as they can transduce a variety of cell types as well as dividing and non-dividing cells. The latter is a game changer for treatment options, as LVs can also infecthematopoietic and T-Cells, cell types that have been proven difficult to transduce for other viral vectors.

However, challenges remain. One that has yet to be overcome is the current demand of LV quantities and required quality for clinical use. Therefore, more efficient production processes for high quality LVs are essential. Here, we show how the upstream section of VSV-G enveloped LV production processes in HEK293 suspension cells can be tweaked to help overcome these limitations.