High Throughput Screening (HTS) or Single-Column Approach for Improvement of Empty/Full Separation of AAV

Accelerate AAV development with a fast, reproducible polishing workflow that separates empty and full capsids across three scalable CIM^® monolith formats – from 96-well plates to CIM^® Octa and single-column runs. The CIM QA HR line ensures consistent elution conductivities and standout resolution, delivering superior enrichment and high recovery. The choice of monolith for screening depends on specific laboratory needs and available equipment.

In this study, we present three different approaches using CIM^® monolith technology for initial screening of conditions for full enrichment:

• High Throughput Screening with 96-Well Plates: This approach allows parallel processing of up to 96 samples, significantly accelerating the development timeline and providing valuable data for scaling up to larger volumes. The plates can be operated manually using a vacuum manifold, positive pressure manifold, centrifuge, or with a fully automated robotic system.
• CIM^® Octa columns: These miniaturized eight-in-line columns are designed for automatic and parallel chromatographic screening of process development parameters. They are operated with a fully automated robotic liquid handling workstation.
• Single Column: This widely accepted method involves screening with a single column, providing a reliable approach to process development