See the wood for the trees: A holistic route to an optimized AAV production process

The promise to provide long lasting therapeutic effects, especially for monogenetic diseases, has resulted in over 2000 viral-based therapeutics projects in the pre-clinical and clinical pipelines. The vast majority of viral vectors is presented by adeno associated virus (AAV) expressed by HEK293 as preferred host. Viral vector manufacturers are facing unique challenges to reduce time-to-market and cost of goods sold (COGS), as viral expression processes are still highly variable.

Optimizationmust consider diverse factors like cell line, serotype, plasmids, cell culturemedia, transfection conditions and the cultivation process as well as complex analytics to characterize the final product. In this work, we present a “Design Space” approach, addressing these key process variables. Our holistic approach facilitates, accelerates, and increases the chance of selecting the bestmedia and processes forAAV production.