Chromatographic Purification of LNP-based Formulations with CIMmultus^® OH

Explore our method guide detailing the chromatography-based purification of lipid nanoparticles (LNPs) using CIMmultus^® OH monolithic column. Designed for large, shear-sensitive molecules, CIMmultus^® columns offer fast flow rates and minimal shear stress, ideal for delicate LNP purification. This method efficiently binds LNPs on CIMmultus^® OH, achieving buffer exchange, ethanol removal, and LNP concentration in a single run. Compared to traditional techniques like TFF and dialysis, it provides higher recoveries, enhanced stability, uniform size distribution, and faster processing, while allowing column reuse to reduce costs.