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The Journey to AAV Production in Suspension – Scaling-Up Your Process
The efficient production of adeno-associated viral vectors (AAVs) starts with scalable upstream manufacturing processes. Here, we present a simple protocol for scaling up AAV production using HEK293 host cells cultured in suspension and under serum-free conditions. We tested cell viability and the genomic titer of AAV-8 and AAV-2 serotypes.