Unlocking Efficiency in LNP Development: Cutting-Edge Tools for Characterization and In Vitro Screening

In the rapidly evolving field of lipid nanoparticle (LNP) research, the integration of advanced technologies is crucial for the efficient generation, screening, and characterization of formulations. This application note highlights a streamlined workflow of innovative techniques used to develop and assess LNP formulations. Utilizing the Sunscreen^® high-throughput screening (HTS) equipment alongside the LipidBrick^® Library, which features a unique collection of proprietary cationic lipids, researchers can achieve efficient formulation development. The note also details the characterization of LNPs using the new Stunner AF^® (Add Fluorescence), which offers high-throughput and accurate analysis of particle size and encapsulation efficiency (EE). Additionally, it covers the in vitro screening of LNP functionality using the Incucyte^® Live-Cell Imaging System, enabling thorough evaluation of transfection efficiency across various cell lines and immune cells. This approach facilitates the optimization of formulations tailored to specific cell types and primary T cells. We explore how these advanced tools and methodologies are transforming LNP research and formulation development.