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Harvesting E. coli for plasmid DNA using single-use, hollow-fiber TFF
Efficient E. coli cell harvesting can support greater scalability and process efficiency in plasmid DNA production. This application note explores single-use hollow-fiber TFF as an alternative to centrifugation, and shows how optimizing key process parameters can help balance performance, fouling, and shear. See how our Sartoflow® 5000 SU system supports a robust and scalable approach to cell harvest.
Key takeaways:
- Benefit from high automation and reduced manual intervention.
- Avoid cleaning-in-place requirements with a single-use design.
- Leverage consistent operation in a closed system and improve biomass quality downstream.
- Align with GMP manufacturing, even in flexible and single-use production environments.
