The Tested Protocol to Scale Up AAV Production With HEK293 Host Cells

Many scientists use adeno-associated viral vectors (AAVs) for gene therapy applications. Robust AAV manufacturing relies on productive host cells, and HEK293 are a common first choice. Transitioning HEK293 cells to suspension culture is your first step toward scalable AAV production.

In this application note, we demonstrate a protocol for producing two AAV serotypes. Read on for our procedure details and results on cell viability.