Exploring Chromatographic Parameters for Enhanced Full AAV Enrichment With CIMmultus PrimaT

This application note highlights how different process parameters affect the performance of the CIMmultus^® SO3 column in the AAV capture step. 

CIMmultus^® SO3 is a strong cation exchange column that:

• Binds any AAV serotype, including engineered capsids 
• Enables fast processing with high recovery and purity 
• Is reusable, supporting cost-efficient manufacturing

Given the diversity of AAV capsids, screening various chromatography conditions is often required to achieve optimal purification results with a specific AAV serotype or to demonstrate the robustness of the selected purification method. When screening is required, understanding the impact of different process parameters is crucial.