Unisart® Nitrocellulose Blotting Membranes
Unisart® Nitrocellulose Blotting Membranes deliver high sensitivity and consistent performance for a wide range of blotting applications and IVD immunoassays. They provide a dependable surface for immobilizing proteins, glycoproteins, and nucleic acids, and support multiple detection methods, such as colorimetric, chemiluminescent, and fluorescent techniques. This versatility makes them well-suited for Western, Southern, and Northern blotting, as well as line, dot, and slot blots.
PFAS-free by design, Unisart® Nitrocellulose Membranes offer a sustainable and eco-friendly solution for your laboratory and IVD diagnostic needs.
Unisart® Nitrocellulose Blotting Membranes
Benefits
Due to their high protein-binding affinity and exceptionally low background, Unisart® Blotting Membranes provide robust protein immobilization and minimal background interference, ensuring consistent and reliable results in your experiments.
Unisart® Nitrocellulose Blotting Membranes offer high membrane stability for fast and easy processing. They are optimized for all protein blotting systems, including electrotransfer, semi-dry and capillary blotting. Additionally, they facilitate easy dispensing with both contact and non-contact equipment.
Unisart® Nitrocellulose Blotting Membranes are compatible with colorimetric, chemiluminescent, and fluorescent detection methods, offering flexibility for various research needs. Their compatibility with various staining and detection protocols makes these membranes an excellent choice for blotting and immunoassays.
Unisart® Nitrocellulose Blotting Membranes do not require any activation step, simplifying your workflow and saving valuable time.
Unisart® Nitrocellulose Blotting Membranes are manufactured without the intentional use of PFC, PFAS, PFOA, PFOS or PTFE making them a sustainable and eco-friendly solution for blotting applications.
All Unisart® Membranes are developed and produced in Göttingen, Germany with adherence to our ISO 9001 certified Quality Management System. All products undergo rigorous in-house testing to meet our stringent quality standards.
Resources
Product resources
Frequently Asked Questions
Unlike PVDF, Unisart® Nitrocellulose Blotting Membranes do not inherently contain fluorinated derivatives. Per- and polyfluoroalkyl compounds, also known as PFC, PFAS, PFOA, PFOS or PTFE are not intentionally used in the manufacturing process. Therefore, Unisart® Nitrocellulose Blotting Membranes offer a sustainable and environmentally friendly solution for blotting applications, aligning with eco-conscious laboratory practices.
Unisart® Nitrocellulose Blotting Membranes are primarily used for protein and nucleic acid transfer in Western, Southern, and Northern blotting applications. They provide high protein-binding affinity and are compatible with colorimetric, chemiluminescent, and fluorescent detection methods. They are also suitable for dot-blot assays, providing excellent signal-to-noise ratios for protein and nucleic acid detection.
The choice of pore size depends on the molecular weight of the target protein being studied. The 0.45 µm pore size is recommended for most analytical blotting experiments, especially for larger proteins. The 0.22 µm pore size is particularly suited for transferring low-molecular weight proteins (<10-15 kDa) and nucleic acids that might pass through larger membrane pores, thereby minimizing sample loss.
No, nitrocellulose membranes do not require activation before use. They are ready to use without any pre-wetting or activation step, making them convenient and easy to integrate into your workflow.
It is recommended to store Unisart® Nitrocellulose Blotting Membranes at a constant temperature between 15–25 °C and a relative humidity between 40 – 60 %. Keep the membranes away from open flames, heat sources, light, and chemical vapors of any kind.
If stored according to our storage recommendations, Unisart® Blotting Membranes have a shelf-life of three years after their manufacturing date. The shelf-life is indicated on each Certificate of Analysis.
The paper separator plays a critical safety role and must remain in place on membrane rolls or sheets until processing. It helps reduce the decomposition rate of Unisart® membranes, which is why all Unisart® membranes are supplied exclusively with this protective separator.
Sartorius offers 0.2 µm and 0.45 µm Unisart® Nitrocellulose Blotting Membranes with a broad range of different formats. All products are available on the Sartorius eShop and can be ordered online. If your desired format is not included, please contact our team via unisart@sartorius.com for more information about customized dimensions.
