Webinar Mastering Monolith Chromatography with DOE

Mastering Monolith Chromatography with DOE: Strategies for Lentiviral Vector and Exosome Purification

Join us for an insightful webinar on the latest advancements in monolith chromatography for lentiviral vector and exosome purification. Discover how Design of Experiments (DOE) can streamline your purification processes, ensuring high purity and infectivity.

We'll explore:
• The impact of starting salt concentration on lentiviral recovery using CIM QA 0.05 mL Monolithic 96-well Plates.
• Key factors and conditions for optimizing downstream purification on the QA column.
• The potential of monoliths in scalable and cost-effective downstream solutions.

Additionally, learn about our innovative approach to optimizing the capture step of extracellular vesicles using the OH column as a substitute for the TFF step. We'll share insights from our screening study, including:
• A wide array of binding conditions tested for the OH column.
• Techniques used to analyze recoveries and purities.
• How DOE software predicts optimal conditions and the results of our validation run.

What You Will Learn:

Discover how Design of Experiments (DOE) can streamline your purification processes
Learn about the potential of monoliths in scalable and cost-effective downstream solutions
Keep abreast with the latest advancements in monolith chromatography for lentiviral vector and exosome purification

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Presenters

Maja Leskovec, BSc

Maja Leskovec leads Process Development for Viruses at Sartorius BIA Separations and has more than 10 years of experience in the industry. She has worked as an application scientist in purification of different viruses and has led several projects with clients in preclinical or clinical stages, all utilizing monolith chromatography as the main purification tool.

Luka Bevc, PhD

Luka Bevc earned his bachelor’s and master’s degrees in Biochemistry from the University of Ljubljana and his PhD in Molecular Mechanisms of Disease from the University of Copenhagen. He works as a Scientist in the Department for Process Development for Viruses at Sartorius BIA Separations, specializing in downstream process development for enveloped viruses. His current focus is on the development and optimisation of downstream process for lentiviruses.

Andrej Raspor, MSc

Andrej Raspor earned his bachelor's degree in Biology and his master’s degree in Biotechnology from the University of Ljubljana. He works as a Scientist in the Department for Process Development for Viruses at Sartorius BIA Separations, specializing in downstream process development for extracellular vesicles.