High Productivity Drives Cost Effectivity at Capture Chromatography for Biologics Manufacturing

For several decades, resins with protein A ligands have been the workhorse of monoclonal antibody (mAb) capture, as their application typically results in >95% purity. Protein A media effectively reduces most host cell protein (HCP) and DNA. However, it is increasingly recognized in the industry that resin-based chromatography matrices are associated with drawbacks, such as value-added pricing of protein A media due to limited supplier options, lower productivity (around 10-20 g/L.h in single column batch mode), which additionally leads to higher cost, ineffective lifetime utilization (particularly pre-commercial), and higher risk.

An alternative approach is to intensify this step by employing membrane-based Rapid Cycling Chromatography (RCC), which relies on an open pore matrix and convective mass transfer to reduce cycle times significantly. Innovative solutions such as RCC provide shorter return on investment times, less risk for bioburden events and significant shorter times to develop a process.


What Will You Learn:


  1. Membrane-based Rapid Cycling Chromatography (RCC) improves process economics
  2. The “one-batch, one-membrane approach” reduces risk and minimizes hands-on time
  3. Critical quality attributes and scalability are maintained, while flexibility is improved using Sartobind® Rapid A

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Ricarda A. Busse

PhD, MBA, Product Manager Chromatography Consumables, Sartorius

Dr. Ricarda A. Busse joined Sartorius in February 2018 as a Product Manager for Membrane Chromatography. She has a PhD in biology | biochemistry from the Georg-August University of Goettingen. She also holds an MBA from the European Fernhochschule Hamburg in General Management, where she specialized in digital and international marketing. She has 8+ years of experience in the biotechnology and bioprocessing industry. Prior to joining Sartorius, she worked as Product and Marketing Manager for affinity chromatography solutions used for recombinant proteins at IBA Lifesciences. During her time as a doctoral candidate at the Max Planck Institute of Biophysical Chemistry, Geottingen, she worked on upstream and downstream process optimization of recombinant proteins from bacterial,

Ganesh Kumar

MS, Integrated Solutions Manager, Sartorius

Ganesh started at Sartorius in 2016 as a Process Engineer | Consultant and was one of the key contributors to the development of the conceptual design platform to design end-to-end process platforms in SU facilities. Today he fills the role of Integrated Solutions Manager. As part of the Integrated Solutions team at Sartorius,

he actively works with clients to conceptualize, design, and deliver process solutions and platforms at clinical | commercial manufacturing stage for production of protein-based and cell | gene-based therapies. He holds a Master of Science in Bioprocess Engineering from Technical University Hamburg-Harburg, Germany. Ganesh started his career at Lonza, Singapore, where he was actively involved in the tech transfer, validation, and large-scale commercial manufacturing of blockbuster mAbs. Over the years, he has actively collaborated|led projects on process positioning, acquisitions, building the technical platform, solution packages and establishing strategic customer collaborations for process intensification. He has over nine years of experience in the biopharmaceutical industry, both as an end-user and a solution provider. He is based in Göttingen, Germany.