Viral Vector Manufacturing in HEK-293 Suspension-Based Platform

Adeno-Associated Viral vectors (AAV) are among the most widely used vectors for gene therapy applications. While cultivation systems such as roller bottles, CellSTACKs (Corning), CellFactories (Nunc, ThermoFisher Scientific), fixed bed reactors, or microcarrier-based cell culture are extensively used in large-scale adherent production of - viral vectors such as AAV, there are many reasons to adapt your process to suspension: Eliminate the need for serum and all its associated negative aspects (animal-origin, Transmissible Spongiform -Encephalopathy | Bovine Spongiform Encephalopathy-risk, costs, ethics, lot-to-lot variation, sourcing) - Allow flexibility regarding scale and volumes per process - Scalable process offers lower operation costs compared to multiple parallel processes - Using controlled cultivation systems for controlled processes generates the potential for optimization Record and understand the process and enable easy documentation of parameters for regulatory purposes.