Optimization of Transient rAAV Production via HEK293 Cell Line Generation, Clone Selection and Production Process

Early-stage process optimization is a critical step in the development of productivity-driven protocols for recombinant adeno associated virus (rAAV) production. During this stage, the cell line is developed and key parameters including media, feeds, plasmid and transfection ratios are tested to determine optimal concentrations and conditions for vector production. Sartorius experts have received the Outstanding Poster Award from the American Society for Cell and Gene Therapy (ASGCT) for their research in this area, specifically as it pertains to HEK 293 cell line generation and rAAV process optimization. Download this poster to explore their findings.

Key Takeaways:

• Learn about a stepwise approach to clone generation, selection, and process optimization
• Explore how a high-throughput single cell cloning approach simplifies clone selection
• Understand how media, transfection reagents and plasmids ratios impact AAV vector productivity including yield and Empty:Full ratio