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End-To-End Process for Improved Purity and Analytics of LNP-Based Therapeutics
The mRNA-LNP production process involves four key steps: in most of the cases expressing plasmid DNA in E. coli, linearizing and purifying the pDNA, synthesizing mRNA through in-vitro transcription, purifying the mRNA, and encapsulating nucleic acids into lipid nanoparticles (LNPs) for effective delivery of mRNA vaccines and therapies.