Fast HPLC Analytics for IVT Reaction Characterization and Tracking Using CIMac PrimaS^™ and PATfix^®

Tracking of IVT Reaction by Rapid "At-Line" PATfix^® HPLC Platform

HPLC with convective chromatography media (e.g. monolith) offers a rapid analytical method to characterize complex biomolecular mixtures. Transcription reaction used for production of mRNA represents such a mixture, with biomolecular components varying in size, chemical and physical properties. A new PATfix^® analytical HPLC approach presented here uses CIMac PrimaS™ to separate IVT components such as NTPs, capping reagent, enzymes, DNA template and mRNA in a very short gradient, opening the door for fast “at-line” tracking.
The study shows how to determine the sample biomolecular components, track the IVT reaction kinetics and define its time course.