June 26 – 29, 2019
Los Angeles Convention Center
Los Angeles, CA
Visit our booth at ISSCR 2019 to learn about our innovative platforms and products.
The IncuCyte® Live-Cell Analysis System automatically acquires and analyzes images around the clock, providing an information-rich analysis that is easy to achieve. Monitor stem cell reprogramming and differentiation events from your desktop without removing cells from the incubator. Identify colonies with IncuCyte® HD-phase contrast imaging and track colony formation, forward or backward in time.
CompacT SelecT™ is the leading automated cell culture system, used in labs across commercial and academic life science. It is now available with new options to enable the culturing of complex cell lines for oncology programs, stem cell research and toxicity studies.
arium® – our laboratory-grade water purification systems feature an inspiring, application-oriented design. They enable you to perform your workflows faster and more reliably, simplifying your daily lab work while ensuring cost-efficient operation over the long term.
Picus® and Tacta Pipettes (link to https://www.sartorius.com/en/products/pipetting-and-dispensing/pipettes) – Our electronic pipettes are ideal tools to accompany you in daily, repetitive liquid handling, when the accuracy of your results, speed and ergonomics are mission-critical. Our mechanical pipettes provide you with ease of use and comfort in your manual pipetting tasks. You may choose the pipette of your liking from the various models we offer to suite your specific needs and applications.
At the show, please visit our poster presentation.
Title: Quantitative Live-cell Analysis Characterizing Morphology And Function Of Ipsc-derived Neurons And Support Cells
Presenter: John Rauch, Scientist Biology and Cell Imaging
Poster board number: T-3212
Poster session: Poster II - Even
Presentation start time: 19:00
Date: Thursday June 27, 2019
Recent advances in stem cell technologies offer an exciting alternative to rodent models for investigating the human nervous system and neurological disorders. The ability to simultaneously monitor morphology and functional readouts over time is critical for thorough characterization of human induced pluripotent stem cell (hiPSC)-derived models. Typically endpoint assays of subtype-specific surface marker expression and morphological features are employed. These techniques have proven valuable, but they fail to couple insight into the ability of these cells to exhibit neuronal activity and develop mature networks. In the case of hiPSC-derived microglia, it is important to ensure that these cells are capable of critical neuroimmune functions such as phagocytosis and chemotaxis. Here we present live-cell imaging data used to quantify functional readouts of hiPSC-derived neurons and microglia and qualitatively assess morphology using the IncuCyte® for Neuroscience. Monitoring of differentiating neuro-progenitor cells (Axol Bioscience) enabled the maturation to iPSC-derived neurons to be visualized over >80 days, with marked morphological and functional development. Activity of hiPSC-derived glutamatergic and GABAergic neurons (CDI, iCell Neurons) was visualized and analyzed using the Incucyte® NeuroBurst reagent, a genetically encoded calcium indicator. While both neuronal types exhibited spontaneous activity, only glutamatergic neurons developed coordinated synaptic network activity over time in culture. To exemplify the benefit of long term monitoring of these models, the CNS.4U® (NCardia) co-culture model of hiPSC-derived neurons and astrocytes were evaluated for over 40 days. While spontaneous activity was detected after one week, correlated network bursting was only observed after 30 days in culture. Finally, hiPSC-derived microglia (Axol Bioscience) were monitored for morphological changes following differentiation and evaluated for phagocytic potential by use of a pH sensitive dye. Collectively, these data illustrate the ability to monitor and characterize hiPSC-derived neuronal cultures and support cells over time using live-cell analysis techniques.