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Sartobind STIC® PA

Sartobind STIC® PA (primary amine) anion exchange membrane overcomes reduced binding capacity for host cell protein removal and can be used in high-salt conditions.

Sartobind STIC PA® 96-Well Plates
Sartobind STIC PA® 96-Well Plates
Item no.: 99STPA42GC-----V

Sartobind STIC® PA (primary amine) anion exchange membrane overcomes reduced binding capacity for host cell protein removal and can be used in high-salt conditions.

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Sartobind STIC® PA 400 mL, 4 mm bed height

Sartobind STIC® PA (primary amine) anion exchange membrane overcomes reduced binding capacity for host cell protein removal and can be used in high-salt conditions.

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Sartobind STIC® PA pico 0.08 mL
Sartobind STIC® PA pico 0.08 mL
Item no.: 92STPA42DD-11--D

Sartobind STIC® PA (primary amine) anion exchange membrane overcomes reduced binding capacity for host cell protein removal and can be used in high-salt conditions.

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Sartobind STIC® PA 75 mL, 4 mm bed height

Sartobind STIC® PA (primary amine) anion exchange membrane overcomes reduced binding capacity for host cell protein removal and can be used in high-salt conditions.

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Sartobind STIC® PA 200 mL, 4 mm bed height

Sartobind STIC® PA (primary amine) anion exchange membrane overcomes reduced binding capacity for host cell protein removal and can be used in high-salt conditions.

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Sartobind STIC® PA removes contaminants at high salt. Salt-tolerant interaction chromatography (STIC) with PA ligand is based on anion-exchange chromatography (AEX) principles. AEX is the established method for removing process-derived contaminants such as host-cell proteins (HCP), DNA, endotoxins, and adventitious and endogenous viruses. It is performed during the downstream processing of monoclonal antibodies (mAbs) in flow-through mode. The process is driven by throughput and not by mass, and thus membrane chromatography (MC) is advantageous over columns for contaminant removal, as membranes run up to two orders of magnitude higher flow rates. Membranes are easy to operate and can be disposed of after one use, just like filters, to save on validation cost.

Applications:

Current mAb downstream processes consist of at least one initial capture and one AEX chromatography step. The limitation of AEX steps is often that the Q chemistry requires low-salt conditions. In processes with cation exchange (CEX) as the second chromatography step, the CEX pool needs to be diluted to conductivities of 4-7 mS/cm to reestablish binding conditions for the following AEX step. The Sartobind STIC PA membrane is composed of cross-linked, regenerated macro-porous cellulose. The primary amine ligand is attached to the matrix at approximately six-fold higher ligand density than that of Q membrane. Both the free amine and the high ligand density were crucial for developing its high salt tolerance.

Benefits:

The Sartobind STIC® adsorber speeds up the production process by high flow rate and opens new opportunities for the purification of therapeutic proteins and vaccines.

  • No dilution of CEX pools necessary
  • Host cell proteins up to 10kg/l (product load/liter membrane)
  • Viruses >4 log
  • DNA at up to 1.5 M NaCl
  • Endotoxins >4 log

Easy to use:

  • Plug and play devices from 0.08 ml to 1.6 L
  • Can be used like a filter
  • Flow rate up to 30 bed volumes/min
  • Disposable after one use

Application Note

Using the Sartobind® pico — Optimizing Steps for using micro-scale membrane adsorbers on liquid chromatography systems
en 1.5 MB
Effect of Phosphate on Binding to Sartobind STIC® PA — Salt Tolerant Membrane Chromatography
en 646.0 kB
Host cell protein removal with Sartobind STIC® PA pico — Performance of salt tolerant anion exchanger in a miniaturized capsule format
en 684.1 kB
Scale up with Sartobind® STIC PA pico — Miniaturized scaleable format for process developers
en 335.7 kB
Host cell protein removal — A comparison between Sartobind STIC® PA and Sartobind Q
en 553.7 kB
High Throughput Screening using Membrane Chromatography — Membrane based 96-well plate for process development in biomanufacturing
en 1.0 MB

Brochure

Sartobind® Membrane Adsorbers — Chromatography as easy as filtration
en 3.7 MB

Datasheet

Sartobind® 96-well plates — High Throughput Screening with IEX and HIC Membrane Chromatography
en 843.2 kB
Sartobind® pico 0.08 mL — Membrane chromatography tool for process development
en 231.9 kB
Sartobind STIC® PA, 4 mm bed height — Salt Tolerant Membrane Adsorbers Anion Exchange
en 1.5 MB

Flyer

Sartobind® selection guide
en 1.0 MB

User Manual

Void Volume Optimized Capsules and Cassettes With 4 mm Bed Height
en 371.5 kB
Sartobind® 96-well plates — IEX (Q, S, STIC PA) and HIC (Phenyl) in 8-strips — For High Throughput Screening Based on Macroporous Membranes, 0.8 mm Bed Height
en 300.6 kB
Manual Sartobind pico 0.08 mL
en 686.8 kB
Sartobind® Selection Kit — pico 0.08 mL with Q, S and STIC PA membranes — A Separation Technology Based on Macroporous Membranes, 4 mm Bed Height
en 396.6 kB