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Octet® Protein L (ProL) Biosensors

Octet® Protein L (ProL) Biosensors are designed for rapid and direct quantitation of a broad set of kappa light chain containing IgG proteins, including whole molecules, FAb fragments and single chain variable fragments, in buffer or complex matrices. Octet® ProL Biosensors are offered in a tray format. Each tray contains 96 biosensors. Larger orders include discounts. The 5-tray pack represents a 10% per tray discount; 20-tray case represents a 20% per tray discount.

Key Features of Octet® Protein L (ProL) Biosensors

  • Broader recognition of Ig isotypes and species compared to Protein A or G
  • Regenerable and cost-effective
  • Binds whole molecule Ab and FAb fragments
  • Quantitate in crude matrices

Top Applications for Octet® Protein L (ProL) Biosensors

  • Quantitation of IgG’s via kappa light chain
  • Hybridoma screening
  • Clone selection
  • Antibody and Fab fragment titer measurement

Item no.:  18-5085

Price (USD):  $645.00

1 Piece
 

Octet® ProL Biosensors are coated with recombinant Protein L, which recognizes a wider range of antibody classes than both Protein A and Protein G, including IgG, IgM, IgA, IgE and IgD. These biosensors offer a rapid and direct method for quantitation of a broad set of kappa light chain containing IgG proteins, including whole molecules, FAb fragments and single chain variable fragments. Octet® Protein L (ProL) Biosensors are especially useful for quantifying antibodies and antibody fragments from serum-based cultures because Protein L does not bind bovine immunoglobulins, which often contaminate serum supplements.

(Note that Protein L does not recognize all kappa light chains. For example, Protein L recognizes mouse VKl (but not VKll, VKIII and VKIV) and human VKI, VKIII and VKIV (but not VKII).)

Key Specifications

  • Typical dynamic range for Fab: 0.05 - 2000 µg/mL
  • Sensitivity can be extended by longer read times and higher shake speed
  • Throughput: 16 and 96 samples in as little as 2 minutes on Octet® RH16 and RH96 respectively
  • Precision: CV < 10%

Cost-Effective Regeneration

Octet® ProL Biosensors can be regenerated up to 10 times via a standard low pH protocol allowing for additional analyses and providing a cost-effective format for analyzing large sample libraries.

Datasheet

Octet® ProL Biosensors Datasheet
en 570.5 kB

Applications

  • Application
    Quantitation

Compliance Information

  • GMP Kits
    No

Features

  • Device/Instrument Compatibility
    • Octet® R8
    • Octet® R4
    • Octet® R2
    • Octet® RH96
    • Octet® RED96e
    • Octet® RH16
    • Octet® N1

Product Information

  • Brand
    Octet®BLI
  • Pack Size
    1
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