Sartobind® Q Mini 10 mL

Sartobind® Q strong ion exchangers are perfect tools for the polishing of monoclonal antibodies for the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins. As size exclusion effects are neglectible, they are suited for purification of viruses such as adeno or lentiviruses displaying one order of magnitude higher dynamic binding capacity.

Catalog No.: 
96IEXQ42D4R11--A

*Custom/bulk order quotes are provided within 72 hours of request.

Sartobind® Q membrane adsorbers display a macro-porous structure with pore size of > 3 µm. Quaternary ammonium ligands are bound covalently to the complete internal surface of the membrane, resulting in separation media of high binding capacity combined with exceptional high flow rates.

Applications virus and VLP capture:

As size exclusion effects are negligible, they are suited for purification of viruses and display one order of magnitude higher dynamic binding capacity compared to resin. Viruses such as adeno- or lentiviruses are captured at high flow rates. The material allows also >100 cycles of re-use without loss of binding capacity.

Benefits capture:

  • High speed purification at 5 - 30 bed volumes per minute
  • No size exclusion effects for large proteins an viruses / VLPs
  • >10fold higher binding capacities compared to conventional resins

Applications flow-through polishing:

Sartobind Q is the perfect tool for the flow-through polishing of valuable proteins such as monoclonal antibodies. The bed volumes can be kept sufficiently small during the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins from such pharmaceutical proteins.

Benefits flow-through polishing:

  • Virus clearance > 6 log
  • DNA below detection limit
  • Host cell protein removal > 99 %
  • Endotoxin removal > 5 log
  • Save 80 % of column costs

Easy to use:

  • Plug and play devices from 0.08 mL to 5 L
  • Can be used like a filter
  • Flow rate up to 30 bed volumes/min
  • Re-usable or disposable

Applications

  • Application
    Polishing

Compliance Information

  • Biological Reactivity
    All materials of this filter element meet the requirements of the current USP Biological Reactivity tests for plastics Class VI (Systemic Injection, Intracutaneous and Implantation tests).

Connections (Physical)

  • Connectors
    Luer female

Dimensions

  • Bed Height
    4 mm
  • Dimensions WxDxH
    70 x 54.5 cm
  • Membrane Area
    364 cm²

Features

  • Reusable
    Yes

Materials of Construction

  • Housing Material
    Polypropylene (PP)
  • Materials of Construction
    Polypropylene; Polyester
  • Membrane Material
    Stabilized reinforced cellulose

Operating Conditions

  • Maximum Operating Pressure
    0.4 MPa
  • Maximum Venting Pressure
    0.5 bar, 0.05 MPa, 7 psi
  • Recommended Flow Rate
    200 mL/min
  • pH Stability
    pH 2 – 14 (short term)

Physicochemical Information

  • Bed Volume
    10 mL
  • Chemical Compatibility
    Stable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents
  • Ligand
    Quaternary ammonium
  • Ligand Density
    2 – 5 µeq/cm²
  • Pore Size
    3 – 5 µm
  • Reference Protein
    Bovine serum albumin (BSA)
  • Thermal Stability
    Sartobind® capsules with ion exchange membranes can be autoclaved at 121 °C for 30 min. We recommend one autoclaving cycle only.
  • Typical Dynamic Binding Capacity 10% per unit
    0.29 g
  • Void Volume
    32 mL

Product Information

  • Brand
    Sartobind® Q
  • Membrane Adsorber Format
    Capsules
  • Membrane Type
    Strong cation exchange membrane
  • Pack Size
    4
  • Type
    Membrane adsorber
  • What you receive
    • 4 capsules
    • 8 adapters Luer male to UNF 10-32 female
    • 1 operating instruction
    • 1 certificate
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