Choose your Incucyte® Reagents and Kits
pHrodo Bioparticles® for Incucyte® are sterile fluorogenic reagents ideally suited to a simple mix-and-read, real-time live cell quantification of phagocytosis. The unique pHrodo®-based system exploits the acidic environment of the phagosome to quantify phagocytosis. As pHrodo Bioparticles® for Incucyte® residing in the neutral extracellular solution (pH 7.4) are engulfed by phagocytes and enter the acidic phagosome (pH 4.5 – 5.5), a substantial increase in fluorescence is observed. Application of pHrodo Bioparticles® for Incucyte® to non-phagocytic cells yields little or no fluorescent signal. With the Incucyte® integrated analysis software background fluorescence is minimized. These fully sterilized reagents have been validated for use with the Incucyte® Live-Cell Analysis System and enable real-time evaluation of phagocytic regulation by pharmacological agents as well as genetic and environmental factors.
Datasheet
Safety Datasheet
Applications
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ApplicationPhagocytosis
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Assay TypePhagocytosis/Efferocytosis
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Research AreaCell Function
Biological Information
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Species +/- IsotypeS. Aureus
Features
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Device/Instrument Compatibility
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S3
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SX1
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SX5
- SX5 configured with G/R
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S3
General Specifications
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ColorRed
Product Information
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BrandIncucyte®
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Pack Size1