pHrodo Bioparticles^® for Incucyte^® are sterile fluorogenic reagents ideally suited to a simple mix-and-read, real-time live cell quantification of phagocytosis. The unique pHrodo^®-based system exploits the acidic environment of the phagosome to quantify phagocytosis. As pHrodo Bioparticles^® for Incucyte^® residing in the neutral extracellular solution (pH 7.4) are engulfed by phagocytes and enter the acidic phagosome (pH 4.5 – 5.5), a substantial increase in fluorescence is observed. Application of pHrodo Bioparticles^® for Incucyte^® to non-phagocytic cells yields little or no fluorescent signal. With the Incucyte^® integrated analysis software background fluorescence is minimized. These fully sterilized reagents have been validated for use with the Incucyte^® Live-Cell Analysis System and enable real-time evaluation of phagocytic regulation by pharmacological agents as well as genetic and environmental factors.