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pHrodo® E. coli Bioparticles® for Incucyte®

One vial of 2 mg sterile lyophilized solid sufficient for 100-200 live cell imaging phagocytosis tests in 96-well format.

Item no.:  4615

Price:  $385.00

Availability:  For more information please log in

   

Incucyte® pHrodo® Bioparticles® are sterile fluorogenic reagents ideally suited to a simple mix-and-read, real-time live cell quantification of phagocytosis. The unique pHrodo®-based system exploits the acidic environment of the phagosome to quantify phagocytosis. As Incucyte® pHrodo® Bioparticles® residing in the neutral extracellular solution (pH 7.4) are engulfed by phagocytes and enter the acidic phagosome (pH 4.5 – 5.5), a substantial increase in fluorescence is observed. Application of Incucyte® pHrodo® Bioparticles to non-phagocytic cells yields little or no fluorescent signal. With the Incucyte® integrated analysis software background fluorescence is minimized. These fully sterilized reagents have been validated for use with the Incucyte® live-cell analysis platform and enable real-time evaluation of phagocytic regulation by pharmacological agents as well as genetic and environmental factors.

 

  • Color
    Red
  • Instrument Compatibility
    • S3
    • SX1
    • SX5 configured with G/R
  • Research Area
    Cell Function
  • Species +/- Isotype
    E. Coli

 

  • Application
    Phagocytosis
  • Pack Size
    1