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iQue® Human 4-Plex Apoptosis Kit

The 4-Plex Apoptosis Kit is a multi-parameter assay consisting 4 different apoptosis measurements per well (cell viability, caspase 3/7 activity, Annexin V binding, and mitochondrial depolarization) for a robust determination of apoptosis progression and insight into mechanism of action. The individual reagent components are also available separately for greater flexibility.

Item no.:  90053

Price:  $675.00

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Key Benefits

  • No wash protocol – just add and read for fast, easily automated workflow. Our 4-plex kit is easier to run than some single endpoint kits!

  • Four parameters to deliver rich content, including insight on mechanism of action.

  • The 4 components are available separately so you can pick and choose.

Multiplex with Qbeads PlexScreen kits for richer content.

Principle

  • The activation of Caspase 3 and 7 is detected by the use of NucView 488 Caspase-3/7 substrate, which upon cleavage by activated enzyme, results in an intracellular fluorescent signal.

  • Surface expression of phosphatidylserine is detected by the binding of Annexin-V to the cell surface.

  • Cell viability is determined by the ability of live cells to exclude a DNA binding.

  • Mitochondrial membrane potential is determined by sequestration of a small fluorescent molecule inside the lumen of intact mitochondria with an active membrane potential. Upon mitochondrial depolarization the dye leaks into the cytoplasm and the cell exhibits a decrease in fluorescence.

 

  • Instrument Compatibility
    • iQue® Screener
    • iQue® Screener PLUS
    • iQue®3
  • Laser Configuration
    • Blue-Red
    • Violet-Blue-Red
  • Reagents and Kits Type
    4-Plex Apoptosis
  • Research Area
    Cell Health
  • Target
    Human

 

  • Application
    Apoptosis
  • Pack Size
    1 x 384