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Incucyte® Nuclight Red Lentivirus (EF1a, Bleo)

One vial of lentivirus reagent (>1x106 TU/mL) suitable for transducing cells with a non-perturbing, with a non-perturbing nuclear restricted red fluorescent label driven off an EF1α promoter) . Stable cell lines can be created under puromycin selection.

Item no.:  4478

Price:  $1,685.00

Availability:  For more information please log in

   

Incucyte® Nuclight Red Lentivirus Reagents enable efficient, non-perturbing, nuclear labelling of living mammalian cells. They are compatible with convenient transduction protocols and enable real-time cell counting and the calculation of cell doubling times. Incucyte® Nuclight Lentivirus Reagents provide homogenous expression of a nuclear-restricted mKate2 (red fluorescent protein) in your choice of primary, immortalized, dividing, or non-dividing cells without altering cell function and with minimal toxicity. These reagents are ideal for generating stable cell populations or clones using puromycin selection. The Incucyte® Nuclight Lentivirus Reagents have been validated for use with the Incucyte® Live-Cell Analysis System and can be combined with the Incucyte® Caspase-3/7, Incucyte® Annexin V or Incucyte® Cytotox Dyes for multiplexed measurements of apoptosis and cytotoxicity alongside cell proliferation in a single well.

Virus Description: 3rd generation HIV-based, VSV-G pseudotyped lentiviral particles encoding a nuclear restricted RFP

Promoter: EF-1 alpha

Selectable Marker: Bleomycin

Spectral Properties: Ex (max): 588 nm; Em (max): 633 nm

Datasheet

Incucyte Nuclight Lentivirus Reagents - For Nuclear Labeling of Live Cells
en 3.8 MB

General Specifications

  • Color
    Red

Volumes

  • Volume
    0.6 mL

eShop Dynamic Attributes

  • Instrument Compatibility
    • S3
    • SX1
    • SX5 configured with G/R
  • Pack Size
    1
  • Research Area
    • Cell Proliferation
    • Cell Function
    • Cell Health