Incucyte^® Nuclight Red Jurkats are supplied as 1 mL cryopreserved vials (1.5 x 10⁶ cells/mL in 90% FBS and 10% DMSO) containing a stable population of human T lymphocyte cells expressing the Incucyte^® Nuclight Red Lentivirus, restricted to the nucleus. Parental Jurkat (clone E6.1) cells (ATCC, Cat. No. TIB-152) were transduced with the Incucyte^® Nuclight Red Lentivirus (Cat. No. 4476; EF1α, puromycin). 48 hours after transduction, the cell population was grown in complete growth media containing 1 µg/ml puromycin, for 3 to 5 days, to select for cells expressing Nuclight Red. Following selection the Nuclight Red Jurkats were validated by comparing morphology and chemotactic migration profiles to the parental cell line. The Nuclight Red Jurkats are certified mycoplasma free by ATCC. These cells are fully validated for use with the Incucyte^®Live-Cell Analysis System and are ideal for use in real-time cell counting studies, co-cultures and chemotaxis assays.