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Incucyte® Nuclight Green Lentivirus (EF1a, Puro)

One vial of lentivirus reagent ( >1x106 TU/mL) suitable for transducing cells with a non-perturbing, nuclear restricted green fluorescent label driven off an EF1α promoter. Stable cell lines can be created under puromycin selection.

Top Features of Incucyte® Nuclight Green Lentivirus (EF1a, Puro)

  • Label cells efficiently and homogenously using a nuclear-restricted green fluorescent protein
  • Study long-term changes in proliferation by generating stably expressing cell lines
  • Quantify true cell counts in mono- or co-culture kinetic assays

Popular Applications for Incucyte® Nuclight Green Lentivirus (EF1a, Puro)

  • Nuclear labeling and real-time quantitation of cell proliferation and viability
  • Multiplexed analyses of apoptosis and cytotoxicity assays when combined with other fluorescent reagents
  • immune cell-killing and the interaction of immune cells with target cancer cells

Item no.:  4475

Price (USD):  $1,880.00

 

Incucyte® Nuclight Green Lentivirus offers a sophisticated, easy-to-use method for nuclear labeling and real-time quantitation of cell proliferation and viability.

Incucyte® Nuclight Lentivirus Reagents enable efficient, non-perturbing, nuclear labeling of living mammalian cells. They are compatible with convenient transduction protocols and enable real-time cell counting and the calculation of cell doubling times.

Incucyte® Nuclight Lentivirus Reagents provide homogenous expression of a nuclear restricted tagGFP2 (green fluorescent protein), mKate2 (red fluorescent protein), TagRFP (orange fluorescent protein) or iRFP713 (NIR fluorescent protein) driven by EF-1α promoter in your choice of primary, immortalized, dividing or non-dividing cells. These reagents are ideal for generating stable cell populations or clones using puromycin or bleomycin selection. Furthermore, the Incucyte® Nuclight Lentivirus Reagents can be combined with the Incucyte® Confluence Metric, Incucyte® Annexin V Dyes, Incucyte® Caspase 3/7 Dyes or Incucyte® Cytotox Dyes for multiplexed measurements of apoptosis, cell proliferation (label-free or fluorescently labeled) or cytotoxicity in every assay well.

Presentation, Storage and Stability

Incucyte® Nuclight Lentivirus Reagents are supplied as 0.2 mL or 0.6 mL vials of 3rd generation HIV-based, VSV-G pseudotyped lentiviral particles suspended in DMEM. Lentiviruses should be handled with utmost care and used only by experienced individuals or under direct supervision. Incucyte® Nuclight Lentivirus Reagents are stable for 6 months from date of receipt when stored at -80°.

Recommended Use

Incucyte® Nuclight Lentivirus can be prepared in full media and added directly to plated cells. We recommend an MOI of 3 to 6, depending on the infected cell type. Optional materials such as the cationic polymer Polybrene® can be added to enhance transduction efficiency.

Post-infection, stable cells may be generated using appropriate antibiotic selection. Please refer to our Product Guide for more information regarding recommended use, protocols, guides and safety information.

Datasheet

Incucyte Nuclight Lentivirus Reagents - For Nuclear Labeling of Live Cells
en 3.8 MB

Safety Datasheet

Incucyte® Nuclight Green Lentivirus (EF1a, Puro) Safety Data Sheet
en 189.0 kB

Applications

  • Application
    • Proliferation
    • Cell Health
    • Apoptosis
  • Assay Type
    • Cell Counting
    • Cell Health
    • Proliferation
    • Viability
    • Nuclear Labeling
  • Research Area
    • Cell Proliferation
    • Cell Function
    • Cell Health

Features

  • Device/Instrument Compatibility
    • S3
    • SX1
    • SX5
  • Optical Module
    • Green/Red
    • Green/Orange/NIR

General Specifications

  • Color
    Green

Product Information

  • Brand
    Incucyte®
  • Pack Size
    1

Volumes

  • Volume
    0.6 mL