Incucyte® Neuroprime Kit
A complete solution for performing kinetic, live-cell imaging of 96-well co-culture assays utilizing primary rat cortical neurons and astrocytes. Supplied as 1 vial (0.45 mL) Incucyte® Neurolight Lentivirus (Synapsin promoter), 1 vial (2 x 106 cells) cryopreserved Incucyte® rCortical Neurons, 1 vial (2 x 106 cells) cryopreserved Incucyte® rAstrocytes.
Item no.: 4585
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The Incucyte® Neuroprime Kits contain cryopreserved vials of rat cortical neurons and astrocytes isolated from embryonic stage (E18) Sprague-Dawley rats as well as sufficient Incucyte® Neurolight Red or Orange Lentivirus to perform one 96-well experiment. The lentivirus encodes a red (mKate2) or orange (TagRFP) fluorescent protein regulated by a synapsin promoter that selectively drives expression in neurons and minimizes expression in nonneuronal cell types. After 6 days in co-culture, the neurons form extensive neurite networks enabling the neurotoxic or neuroprotective effects of treatments to be assessed. Dynamic changes in neuronal network length and branching are measured using Incucyte® Live-Cell Analysis Systems.
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Research AreaCell Function