Incucyte® Neurolight Lentivirus (Synapsin)
Two vials (0.45 mL/vial) of Incucyte® Neurolight Lentivirus (synapsin promoter) suitable for transducing a variety of neuronal cell types in a 96-well format.
The Incucyte® Neurolight Lentivirus Reagents are third generation HIV-based, VSV-G pseudotyped lentiviral particles to enable highly-efficient, yet non-disruptive labeling of primary or iPSC-derived neurons. Once integrated into cells of interest, a neuron-specific (synapsin) promoter drives stable, long-term expression of a red or orange fluorescent protein (mKate2 or TagRFP, respectively) in neuronal cell bodies and neurites. This enables the kinetic quantification of neurite length and branching over a period of several weeks, even in the presence of astrocytes and other non-neuronal cell types such as microglia. The Incucyte® Neurolight Red and Orange Lentivirus Reagents have been validated for use with the Incucyte® Live-Cell Analysis Systems for measurements of neurite outgrowth, maturation, and the disruption of neurite networks.
- Neuronal Labeling
- Cell Health