Incucyte® Neuroactive Orange Kit
A complete solution for performing kinetic, live-cell imaging of 96-well assays for sponataneous neuronal activity. Supplied as 1 vial (2 mL) Incucyte® Neuroburst OrangeLentivirus (Synapsin promoter), 1 vial (2 x 106 cells) cryopreserved Incucyte® rCortical Neurons, 1 vial (2 x 106 cells) cryopreserved Incucyte® rAstrocytes.
Item no.: 4761
Price (USD): $1,715.00
The Incucyte® Neuroactive Kit contain cryopreserved vials of rat cortical neurons and astrocytes isolated from embryonic stage (E18) Sprague-Dawley rats as well as sufficient Incucyte® Neuroburst Orange Lentivirus to perform one 96-well experiment.
The Incucyte® Neuroburst Orange Lentivirus is a lentiviral based live-cell neuronal labeling reagent driven by a synapsin promoter, resulting in the long term expression of a genetically encoded orange fluorescent calcium indicator (mRuby-based) in neuronal cells. The Incucyte® Neuroburst Orange Lentivirus ensures highly efficient, yet non-disruptive labeling of primary or iPSC-derived neurons over days and weeks, and enables the kinetic quantification of neuronal activity and functional connectivity in the presence or absence of astrocytes.
The Incucyte® Neuroburst Orange Lentivirus has been validated for use with the Incucyte® S3 Live-Cell Analysis System configured with an Orange/NIR optical module and the Incucyte® SX5 Live-Cell Analysis System (Cat. No. 4816) for measurements of neuronal activity and functional connectivity with the Incucyte® Neuronal Activity Analysis Software Module (Cat. No. 9600-0032). The Neuroburst Orange Lentivirus is NOT compatible with Incucyte instruments configured with a Green/Red Optical Module, e.g. Cat. No. 4647.