Incucyte® Mouse IgG1 Fabfluor Antibody Labeling Dye for Live-Cell Immunocytochemistry
One vial (50 µg) of lyophilized conjugate sufficient for labeling for labeling surface expressed proteins in 96-well format. One vial of background suppressor supplied for Incucyte® Fabfluor-488.
Top Features of Incucyte® Mouse IgG1 Fabfluor Antibody Labeling Dye for Live-Cell Immunocytochemistry
- Fast, easy labeling of Fc- containing test antibodies with a green or red fluorophore
- Identify surface-expressed antigens accurately and reproducibly
- Validated for use with a range of antibodies of varying cell types
Popular Applications for Incucyte® Mouse IgG1 Fabfluor Antibody Labeling Dye
- Live-cell immunocytochemistry
Item no.: 4745
Incucyte® Mouse IgG1 Antibody Labeling Dyes for Live-Cell Immunochemistry are designed for quick, easy labeling of Fc containing test antibodies with a green or red fluorophore. Once labeled, the Fabfluor-antibody complex can be used for identification of surface expressed antigens in live cells. In the absence of expressed specific antigen, little or no signal is seen on the cells. With the Incucyte® integrated analysis software, background fluorescence is minimized. This reagent has been validated for use with a number of different antibodies in a range of cell types. The Incucyte® Live-Cell Analysis System enables real time, kinetic evaluation of live-cell immunocytochemistry. Furthermore, the Incucyte® Mouse IgG1 Dyes can be multiplexed with the other Incucyte® reagents, enabling measurements of different cellular events, protein localizations, or cell types in a single well.
The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. Based on antigen-binding assay and/or ELISA the antibody reacts with the Fc portion, but not the Fab portion of mouse immunoglobulin 1 or 2a. No reactivity is demonstrated against IgM or other non-immunoglobulin serum proteins. The antibody may cross-react with other mouse IgG subclasses or with immunoglobulins from other species.
Presentation, Storage, and Stability
The Incucyte® Mouse IgG1 Fabluor Antibody Labeling Dye is supplied as a lyophilized solid in sufficient quantity to label 50 µg of test antibody (when used at suggested molar ratio 1:3 of a test antibody to labeling Fab). For storage, the lyophilized solid should be stored at 2-8°C, with stability for at least one year. Once rehydrated, it is recommended that the solution is used as soon as possible or aliquoted and stored at -80°C for up to 1 year. Freezing and thawing should be avoided.
Incucyte® Opti-Green background suppressor is supplied with the Incucyte® Mouse IgG1 Fabfluor-488 Dye as 200 µL of 100 mM stock solution in water. The stock liquid should be stored at 2–8° C (stable for at least 1 year). Bring to room temperature and mix well (vortex) before use. Dilute on day of use in assay media.
The Incucyte® Mouse IgG1 Antibody Labeling Dyes should be prepared at a stock concentration of 0.5 mg/mL by the addition of 100 μL of sterile water and triturated (centrifuge if solution not clear). The reagent may then be diluted directly into the labeling mixture with test antibody. The solution should NOT be sonicated.
For Incucyte® Mouse IgG1 Fabfluor-488 Antibody Labeling Dye: We recommend that Incucyte® Opti-Green background suppressor is mixed well before use. Stock solution should be diluted in complete growth media to produce a final assay concentration of 0.5 mM or a 1: in 200 dilution of stock. This has been shown to be suitable across a range of cell types.
Additional protocol details can be found in the Product Guide under the Documents tab.
Assay TypeAntibody Labeling
Species +/- IsotypeMouse IgG1
- SX5 configured with G/R