Incucyte® Cell Cycle Lentivirus (EF1a, Puro)
One vial of lentivirus reagent (0.2 mL; >1x106 TU/mL) suitable for transducing cells with a non-perturbing, selectable cell cycle indicator. Stable cell lines can be created under puromycin selection.
Item no.: 4779
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Incucyte® Cell Cycle Lentivirus Reagents enable efficient, non-perturbing, and homogeneous labeling of living mammalian cells for in vitro analysis of cell cycle progression. The Incucyte® Cell Cycle Lentivirus Reagents are single-cassette, genetically encoded ubiquitination-based indicators that take advantage of cell cycle dependent changes in the expression patterns of Geminin and Cdt1. By linking fluorescent proteins TagGFP2 and mKate2 (green/red), or TagGFP2 and TagRFP (green/orange) to fragments of Geminin and Cdt1, the G1 and S/G2/M phases can be monitored in realtime. These reagents are ideal for generating stable cell populations or clones using puromycin selection. The Incucyte® Cell Cycle Green/Red Lentivirus Reagent is compatible with the Incucyte® Live-Cell Analysis System configured with a Green/Red optical module. The Incucyte® Cell Cycle Green/Orange Lentivirus Reagent is compatible with the Incucyte® Live-Cell Analysis System configured with a Green/Orange/NIR optical module.
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eShop Dynamic Attributes
- SX5 configured with G/R
Research AreaCell Proliferation