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Incucyte® Caspase-3/7 Dye for Apoptosis

One vial (20 µl) of 5 mM mix and read reagent in DMSO sufficient for 100-200 live-cell apoptosis tests in 96-well format.

Item no.:  4440

Price:  $330.00

Availability:  For more information please log in

   

The Incucyte® Caspase-3/7 Dye for Apoptosis couples the activated caspase-3/7 recognition motif (DEVD) to a DNA intercalating dye to enable quantification of apoptosis over time. The Incucyte® Caspase-3/7 Dye is specially formulated for use in the Incucyte® Live-Cell Analysis System and can be added directly to tissue culture wells using a no wash, mix and read protocol to acquire live cell images of cells undergoing caspase-3/7 mediated apoptosis. In addition, the Incucyte® Caspase-3/7 Dye is non-perturbing to cell growth and morphology. When added to tissue culture medium, this inert, non- fluorescent substrate crosses the cell membrane where it is cleaved by activated caspase-3/7 resulting in the release of the DNA dye and fluorescent staining of nuclear DNA.

Kinetic activation of caspase-3/7 can be monitored morphologically using live-cell imaging, and quantified using the Incucyte® integrated software analysis tools. Furthermore, the Incucyte® Casepase 3/7 can be combined with the Incucyte® confluence metric, Incucyte® Annexin V Dyes, Incucyte® Nuclight Reagents for nuclear labeling or Incucyte® Cytotox Dyes for multiplexed measurements of apoptosis, cell proliferation or cytotoxicity in every assay well.

Application Note

Incucyte®️ Caspase 3/7 Dye Product Guide
en 2.0 MB

 

  • Color
    Green
  • Instrument Compatibility
    • S3
    • SX1
    • SX5
    • SX5 configured with G/R
  • Research Area
    Cell Health

 

  • Application
    Apoptosis
  • Pack Size
    1
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