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Incucyte® Caspase-3/7 Dye for Apoptosis

One vial (20 µL) of mix-and-read reagent in DMSO sufficient for 100-200 live-cell apoptosis tests (1 test = 1 well of 96-well microtiter plate) in 96-well format.

Top Features of Incucyte® Caspase-3/7 Dye for Apoptosis

  • Reduce loss of precious or compromised cells under-going caspase-3/7 mediated apoptosis with mix-and-read reagents
  • Track true, artifact-free biological responses with non-perturbing, live-cell reagents
  • Quantify apoptosis in real-time with the Incucyte® Live-Cell Analysis System

Popular Applications for Incucyte® Caspase-3/7 Dye for Apoptosis

  • Immune cell killing
  • Multiplex with proliferation
  • Multi-spheroid growth

Item no.:  4440

Price:  $370.00

 

Incucyte® Caspase-3/7 Dyes for Apoptosis are formulated to detect apoptosis (cell death) in live cells. The dyes are ideally suited to the mix-and-read, real-time quantification of cells undergoing caspase-3/7 mediated apoptosis. Addition of the Incucyte® Caspase-3/7 Green, Red or Metabolism Dyes to healthy cells is non-perturbing to cell growth and morphology. When used with Incucyte® Live-Cell Analysis Systems and software, these reagents allow for reliable, accurate evaluation of cell death. Incucyte® Caspase-3/7 Dyes for Apoptosis couple the activated caspase-3/7 recognition motif (DEVD) to a DNA intercalating dye. When added to tissue culture medium, the inert, non-fluorescent substrate crosses the cell membrane where it is cleaved by activated caspase-3/7, resulting in the release of the DNA dye and fluorescent staining of the nuclear DNA. With Incucyte® integrated analysis software, fluorescent objects can be quantified and background fluorescence minimized. These reagents have been validated for use with the Incucyte® Live-Cell Analysis System and enable real-time evaluation of cell apoptosis induced by pharmacological agents and/or genetic and environmental factors.

Presentation, Storage and Stability

The dyes are supplied as single-vial solutions in dimethyl sulfoxide (DMSO). Each vial provides a sufficient quantity for performing 100-200 tests (one test is one well of a 96-well microtiter plate). Upon receipt, the Incucyte® Caspase 3/7 Dyes should be stored at 4°C where they are stable for six months.

Recommended Use

We recommend diluting Incucyte® Caspase-3/7 Dyes in complete culture media and adding directly to cells in culture, as per the guidelines below:

  • Incucyte® Caspase-3/7 Green: 1:1000 in culture media, for a final assay concentration of 5 μM
  • Incucyte® Caspase-3/7 Red: Optimize final assay concentration by diluting the reagent 1:200 in complete medium, then make a series of 2-fold dilutions for final assay concentrations of 2.5, 1.25 and 0.5 µM
  • Incucyte® Caspase-3/7 for Metabolism: 1:1000 in culture media, for a final assay concentration of 1 μM

Additional protocol details can be found in the Product Guide under the Documents tab.

Application Note

Incucyte®️ Caspase 3/7 Dye Product Guide
en 1.6 MB

Safety Datasheet

Incucyte® Caspase 3/7 Green Dye Safety Data Sheet
en 91.8 kB

Applications

  • Application
    Apoptosis
  • Assay Type
    • Cell Health
    • Viability
  • Research Area
    Cell Health

Features

  • Device/Instrument Compatibility
    • S3
    • SX1
    • SX5
    • SX5 configured with G/R

General Specifications

  • Color
    Green

Product Information

  • Brand
    Incucyte®
  • Pack Size
    1
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One vial of lentivirus reagent (>1x106 TU/mL) suitable for transducing cells with a non-perturbing, nuclear restricted red fluorescent label driven off an EF1α promoter. Stable cell lines can be created under puromycin selection.

Top Features of Incucyte® Nuclight Red Lentivirus (EF1a, Puro)

  • Label cells efficiently and homogenously using a nuclear-restricted red fluorescent protein
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Popular Applications for Incucyte® Nuclight Red Lentivirus (EF1a, Puro)

  • Nuclear labeling and real-time quantitation of cell proliferation and viability
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Incucyte® Cytolight Rapid Dye for Live-cell Cytoplasmic Labeling

$270.00

Supplied as dry powder in sufficient quantity capable of labeling between 10 and 90 million cells depending on the optimized concentration.

Top Features of Incucyte® Cytolight Rapid Dye for Live-Cell Cytoplasmic Labeling

  • Rapid, efficient cytoplasmic labeling of living cells
  • At optimal concentrations, non-perturbing to cell growth and morphology
  • Ideal for labeling cell types that are difficult to label using BacMam, lentivirus or other transfection-based labeling approaches

Popular Applications for Incucyte® Cytolight Rapid Dye for Live-Cell Cytoplasmic Labeling

  • Well-suited for identifying the location of cells within a population and monitoring the dynamic interactions between cells in mixed cultures
  • Immune cell killing assays – Cytotoxic T cell killing, Antibody-dependent cell-mediated cytotoxicity (ADCC), immune cell killing in tumor spheroid models

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One vial of lyophilized solid sufficient for 100-200 live-cell apoptosis tests (1 test = 1 well of 96-well microtiter plate) in 96-well format.

Top Features of Incucyte® Annexin V Dye for Apoptosis

  • Detect apoptosis using a highly-selective phosphatidylserine cyanine fluorescent dye
  • Track relevant biology with a dye that is non-perturbing to cell health and morphology
  • Reduce loss of precious or compromised cells under-going apoptosis with mix-and-read reagents

Popular Applications for Incucyte® Annexin V Dye for Apoptosis

  • Apoptosis/cell death quantification
  • Immune cell killing
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$215.00

Supplied as dry powder in sufficient quantity capable of labeling between 10 and 90 million cells depending on the optimized concentration.

Top Features of Incucyte® Cytolight Rapid Dye for Live-Cell Cytoplasmic Labeling

  • Rapid, efficient cytoplasmic labeling of living cells
  • At optimal concentrations, non-perturbing to cell growth and morphology
  • Ideal for labeling cell types that are difficult to label using BacMam, lentivirus or other transfection-based labeling approaches

Popular Applications for Incucyte® Cytolight Rapid Dye for Live-Cell Cytoplasmic Labeling

  • Well-suited for identifying the location of cells within a population and monitoring the dynamic interactions between cells in mixed cultures
  • Immune cell killing assays – Cytotoxic T cell killing, Antibody-dependent cell-mediated cytotoxicity (ADCC), immune cell killing in tumor spheroid models

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