Incucyte® Caspase-3/7 Dye for Apoptosis
One vial (20 µL) of mix-and-read reagent in DMSO sufficient for 100-200 live-cell apoptosis tests (1 test = 1 well of 96-well microtiter plate) in 96-well format.
Top Features of Incucyte® Caspase-3/7 Dye for Apoptosis
- Reduce loss of precious or compromised cells under-going caspase-3/7 mediated apoptosis with mix-and-read reagents
- Track true, artifact-free biological responses with non-perturbing, live-cell reagents
- Quantify apoptosis in real-time with the Incucyte® Live-Cell Analysis System
Popular Applications for Incucyte® Caspase-3/7 Dye for Apoptosis
- Immune cell killing
- Multiplex with proliferation
- Multi-spheroid growth
Item no.: 4440
Price (USD): $370.00
Choose your Incucyte® Reagents and Kits
Incucyte® Caspase-3/7 Dyes for Apoptosis are formulated to detect apoptosis (cell death) in live cells. The dyes are ideally suited to the mix-and-read, real-time quantification of cells undergoing caspase-3/7 mediated apoptosis. Addition of the Incucyte® Caspase-3/7 Green, Red or Metabolism Dyes to healthy cells is non-perturbing to cell growth and morphology. When used with Incucyte® Live-Cell Analysis Systems and software, these reagents allow for reliable, accurate evaluation of cell death. Incucyte® Caspase-3/7 Dyes for Apoptosis couple the activated caspase-3/7 recognition motif (DEVD) to a DNA intercalating dye. When added to tissue culture medium, the inert, non-fluorescent substrate crosses the cell membrane where it is cleaved by activated caspase-3/7, resulting in the release of the DNA dye and fluorescent staining of the nuclear DNA. With Incucyte® integrated analysis software, fluorescent objects can be quantified and background fluorescence minimized. These reagents have been validated for use with the Incucyte® Live-Cell Analysis System and enable real-time evaluation of cell apoptosis induced by pharmacological agents and/or genetic and environmental factors.
Presentation, Storage and Stability
The dyes are supplied as single-vial solutions in dimethyl sulfoxide (DMSO). Each vial provides a sufficient quantity for performing 100-200 tests (one test is one well of a 96-well microtiter plate). Upon receipt, the Incucyte® Caspase 3/7 Dyes should be stored at 4°C where they are stable for six months.
Recommended Use
We recommend diluting Incucyte® Caspase-3/7 Dyes in complete culture media and adding directly to cells in culture, as per the guidelines below:
- Incucyte® Caspase-3/7 Green: 1:1000 in culture media, for a final assay concentration of 5 μM
- Incucyte® Caspase-3/7 Red: Optimize final assay concentration by diluting the reagent 1:200 in complete medium, then make a series of 2-fold dilutions for final assay concentrations of 2.5, 1.25 and 0.5 µM
- Incucyte® Caspase-3/7 for Metabolism: 1:1000 in culture media, for a final assay concentration of 1 μM
Additional protocol details can be found in the Product Guide under the Documents tab.
Application Note
Safety Datasheet
Applications
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ApplicationApoptosis
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Assay Type
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Cell Health
- Viability
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Cell Health
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Research AreaCell Health
Features
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Device/Instrument Compatibility
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S3
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SX1
- SX5
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S3
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Optical Module
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Green/Red
- Green/Orange/NIR
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Green/Red
General Specifications
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ColorGreen
Product Information
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BrandIncucyte®
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Pack Size1
One vial of 2 mg sterile lyophilized solid sufficient for 100-200 live-cell imaging phagocytosis tests in 96-well format.
4615 and 4616 are on backorder.
One cryopreserved vial of A549 cells expressing Incucyte® Nuclight Lentivirus (nuclear-labeled fluorescent protein) at a density of 1x106 cells/vial.
This product is experiencing longer than normal lead times to customers outside the United States.
One vial of lentivirus reagent (>1x106 TU/mL) suitable for transducing cells with a non-perturbing, with a non-perturbing nuclear restricted red fluorescent label driven off an EF1α promoter) . Stable cell lines can be created under puromycin selection.
One cryopreserved vial of HT-1080 cells expressing Incucyte® Nuclight Lentivirus (nuclear-labeled fluorescent protein) at a density of 1x106 cells/vial.
One cryopreserved vial of HeLa cells expressing Incucyte® Nuclight Lentivirus (nuclear-labeled fluorescent protein) at a density of 1x106 cells/vial.
One vial of lentivirus reagent (>1x106 TU/mL) suitable for transducing cells with a non-perturbing, nuclear restricted red fluorescent label driven off an EF1α promoter. Stable cell lines can be created under puromycin selection.
Top Features of Incucyte® Nuclight Red Lentivirus (EF1a, Puro)
- Label cells efficiently and homogenously using a nuclear-restricted red fluorescent protein
- Study long-term changes in proliferation by generating stably expressing cell lines
- Quantify true cell counts in mono- or co-culture kinetic assays
Popular Applications for Incucyte® Nuclight Red Lentivirus (EF1a, Puro)
- Nuclear labeling and real-time quantitation of cell proliferation and viability
- Multiplexed analyses of apoptosis and cytotoxicity assays when combined with other fluorescent reagents
- Study immune cell-killing and the interaction of immune cells with target cancer cells
This product is experiencing longer than normal lead times to customers outside the United States.
One cryopreserved vial of immortalised human T lymphocyte cells stably expressing a nuclear restricted red fluorescent protein (1.5 x 106 cell/vial).
Supplied as solution in dimethyl sulfoxide (DMSO), providing sufficient quantity for performing 500–1000 tests (1 test = 1 well of 96-well microtiter plate).
Top Features of Incucyte® Cytotox Dye
- Detect cytotoxicity in real time with non-perturbing reagents
- Reduce loss of compromised cells with simple, mix-and-read protocols
- Acquire reliable data with little or no intrinsic fluorescence
- Multiplex with proliferation and apoptosis measurements
Popular Applications for Incucyte® Cytotox Dye
- Ideal for detection of cell membrane integrity disruption and quantitation of cell death in real-time
- Multiplexed applications, such as apoptosis, proliferation, and single spheroid growth, when combined with other Incucyte® reagents and metrics
- NETosis
Supplied as dry powder in sufficient quantity capable of labeling between 10 and 90 million cells depending on the optimized concentration.
Top Features of Incucyte® Cytolight Rapid Dye for Live-Cell Cytoplasmic Labeling
- Rapid, efficient cytoplasmic labeling of living cells
- At optimal concentrations, non-perturbing to cell growth and morphology
- Ideal for labeling cell types that are difficult to label using BacMam, lentivirus or other transfection-based labeling approaches
Popular Applications for Incucyte® Cytolight Rapid Dye for Live-Cell Cytoplasmic Labeling
- Well-suited for identifying the location of cells within a population and monitoring the dynamic interactions between cells in mixed cultures
- Immune cell killing assays – Cytotoxic T cell killing, Antibody-dependent cell-mediated cytotoxicity (ADCC), immune cell killing in tumor spheroid models
One vial of lyophilized solid sufficient for 100-200 live-cell apoptosis tests (1 test = 1 well of 96-well microtiter plate) in 96-well format.
Top Features of Incucyte® Annexin V Dye for Apoptosis
- Detect apoptosis using a highly-selective phosphatidylserine cyanine fluorescent dye
- Track relevant biology with a dye that is non-perturbing to cell health and morphology
- Reduce loss of precious or compromised cells under-going apoptosis with mix-and-read reagents
Popular Applications for Incucyte® Annexin V Dye for Apoptosis
- Apoptosis/cell death quantification
- Immune cell killing
- Multiplexed measurements such as cytotoxicity and proliferation when combined with other Incucyte® products, including Incucyte® Caspase 3/7 Dyes, Incucyte® Nuclight Reagents, Incucyte® Cytotox Dyes and Incucyte® Confluence Metrics
Supplied as dry powder in sufficient quantity capable of labeling between 10 and 90 million cells depending on the optimized concentration.
Top Features of Incucyte® Cytolight Rapid Dye for Live-Cell Cytoplasmic Labeling
- Rapid, efficient cytoplasmic labeling of living cells
- At optimal concentrations, non-perturbing to cell growth and morphology
- Ideal for labeling cell types that are difficult to label using BacMam, lentivirus or other transfection-based labeling approaches
Popular Applications for Incucyte® Cytolight Rapid Dye for Live-Cell Cytoplasmic Labeling
- Well-suited for identifying the location of cells within a population and monitoring the dynamic interactions between cells in mixed cultures
- Immune cell killing assays – Cytotoxic T cell killing, Antibody-dependent cell-mediated cytotoxicity (ADCC), immune cell killing in tumor spheroid models
Supplied as solution in dimethyl sulfoxide (DMSO), providing sufficient quantity for performing 500–1000 tests (1 test = 1 well of 96-well microtiter plate).
Top Features of Incucyte® Cytotox Dye
- Detect cytotoxicity in real time with non-perturbing reagents
- Reduce loss of compromised cells with simple, mix-and-read protocols
- Acquire reliable data with little or no intrinsic fluorescence
- Multiplex with proliferation and apoptosis measurements
Popular Applications for Incucyte® Cytotox Dye
- Ideal for detection of cell membrane integrity disruption and quantitation of cell death in real-time
- Multiplexed applications, such as apoptosis, proliferation, and single spheroid growth, when combined with other Incucyte® reagents and metrics
- NETosis