Incucyte® Caspase-3/7 Dye for Apoptosis
One vial (20 µl) of 5 mM mix and read reagent in DMSO sufficient for 100-200 live-cell apoptosis tests in 96-well format.
Item no.: 4440
Price: $330.00
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The Incucyte® Caspase-3/7 Dye for Apoptosis couples the activated caspase-3/7 recognition motif (DEVD) to a DNA intercalating dye to enable quantification of apoptosis over time. The Incucyte® Caspase-3/7 Dye is specially formulated for use in the Incucyte® Live-Cell Analysis System and can be added directly to tissue culture wells using a no wash, mix and read protocol to acquire live cell images of cells undergoing caspase-3/7 mediated apoptosis. In addition, the Incucyte® Caspase-3/7 Dye is non-perturbing to cell growth and morphology. When added to tissue culture medium, this inert, non- fluorescent substrate crosses the cell membrane where it is cleaved by activated caspase-3/7 resulting in the release of the DNA dye and fluorescent staining of nuclear DNA.
Kinetic activation of caspase-3/7 can be monitored morphologically using live-cell imaging, and quantified using the Incucyte® integrated software analysis tools. Furthermore, the Incucyte® Casepase 3/7 can be combined with the Incucyte® confluence metric, Incucyte® Annexin V Dyes, Incucyte® Nuclight Reagents for nuclear labeling or Incucyte® Cytotox Dyes for multiplexed measurements of apoptosis, cell proliferation or cytotoxicity in every assay well.
Application Note
Safety Datasheet
General Specifications
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ColorGreen
eShop Dynamic Attributes
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ApplicationApoptosis
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Instrument Compatibility
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S3
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SX1
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SX5
- SX5 configured with G/R
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S3
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Pack Size1
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Research AreaCell Health
One vial of 2 mg sterile lyophilized solid sufficient for 100-200 live-cell imaging phagocytosis tests in 96-well format
One cryopreserved vial of A549 cells expressing Incucyte® Nuclight Lentivirus (nuclear-labeled fluorescent protein) at a density of 1x106 cells/vial.
One vial of lentivirus reagent (>1x106 TU/mL) suitable for transducing cells with a non-perturbing, with a non-perturbing nuclear restricted red fluorescent label driven off an EF1α promoter) . Stable cell lines can be created under puromycin selection.
One cryopreserved vial of HT-1080 cells expressing Incucyte® Nuclight Lentivirus (nuclear-labeled fluorescent protein) at a density of 1x106 cells/vial.
One cryopreserved vial of HeLa cells expressing Incucyte® Nuclight Lentivirus (nuclear-labeled fluorescent protein) at a density of 1x106 cells/vial
One vial of lentivirus reagent (>1x106 TU/mL) suitable for transducing cells with a non-perturbing, with a non-perturbing nuclear restricted green fluorescent label driven off an EF1α promoter) . Stable cell lines can be created under puromycin selection.
One vial of lentivirus reagent (>1x106 TU/mL) suitable for transducing cells with a non-perturbing, with a non-perturbing nuclear restricted red fluorescent label driven off an EF1α promoter) . Stable cell lines can be created under puromycin selection.
Supplied as solution in dimethyl sulfoxide (DMSO), providing sufficient quantity for performing 500–1000 tests (1 test = 1 well of 96-well microtiter plate).
One vial of lyophilized solid sufficient for 100-200 live-cell apoptosis tests (1 test = 1 well of 96-well microtiter plate) in 96-well format.
Supplied as dry powder in sufficient quantity capable of labeling between 10 and 90 million cells depending on the optimized concentration.
One vial of lyophilized solid sufficient for 100-200 live-cell apoptosis tests (1 test = 1 well of 96-well microtiter plate) in 96-well format.
Supplied as dry powder in sufficient quantity capable of labeling between 10 and 90 million cells depending on the optimized concentration.
Supplied as solution in dimethyl sulfoxide (DMSO), providing sufficient quantity for performing 500–1000 tests (1 test = 1 well of 96-well microtiter plate).