Incucyte® Caspase-3/7 Dye for Apoptosis
One vial (20 µL) of mix-and-read reagent in DMSO sufficient for 100-200 live-cell apoptosis tests (1 test = 1 well of 96-well microtiter plate) in 96-well format.
Top Features of Incucyte® Caspase-3/7 Dye for Apoptosis
- Reduce loss of precious or compromised cells under-going caspase-3/7 mediated apoptosis with mix-and-read reagents
- Track true, artifact-free biological responses with non-perturbing, live-cell reagents
- Quantify apoptosis in real-time with the Incucyte® Live-Cell Analysis System
Popular Applications for Incucyte® Caspase-3/7 Dye for Apoptosis
- Immune cell killing
- Multiplex with proliferation
- Multi-spheroid growth
Item no.: 4440
Price: $370.00
Choose your Incucyte® Reagents and Kits
Incucyte® Caspase-3/7 Dyes for Apoptosis are formulated to detect apoptosis (cell death) in live cells. The dyes are ideally suited to the mix-and-read, real-time quantification of cells undergoing caspase-3/7 mediated apoptosis. Addition of the Incucyte® Caspase-3/7 Green, Red or Metabolism Dyes to healthy cells is non-perturbing to cell growth and morphology. When used with Incucyte® Live-Cell Analysis Systems and software, these reagents allow for reliable, accurate evaluation of cell death. Incucyte® Caspase-3/7 Dyes for Apoptosis couple the activated caspase-3/7 recognition motif (DEVD) to a DNA intercalating dye. When added to tissue culture medium, the inert, non-fluorescent substrate crosses the cell membrane where it is cleaved by activated caspase-3/7, resulting in the release of the DNA dye and fluorescent staining of the nuclear DNA. With Incucyte® integrated analysis software, fluorescent objects can be quantified and background fluorescence minimized. These reagents have been validated for use with the Incucyte® Live-Cell Analysis System and enable real-time evaluation of cell apoptosis induced by pharmacological agents and/or genetic and environmental factors.
Presentation, Storage and Stability
The dyes are supplied as single-vial solutions in dimethyl sulfoxide (DMSO). Each vial provides a sufficient quantity for performing 100-200 tests (one test is one well of a 96-well microtiter plate). Upon receipt, the Incucyte® Caspase 3/7 Dyes should be stored at 4°C where they are stable for six months.
Recommended Use
We recommend diluting Incucyte® Caspase-3/7 Dyes in complete culture media and adding directly to cells in culture, as per the guidelines below:
- Incucyte® Caspase-3/7 Green: 1:1000 in culture media, for a final assay concentration of 5 μM
- Incucyte® Caspase-3/7 Red: Optimize final assay concentration by diluting the reagent 1:200 in complete medium, then make a series of 2-fold dilutions for final assay concentrations of 2.5, 1.25 and 0.5 µM
- Incucyte® Caspase-3/7 for Metabolism: 1:1000 in culture media, for a final assay concentration of 1 μM
Additional protocol details can be found in the Product Guide under the Documents tab.
Application Note
Safety Datasheet
Applications
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ApplicationApoptosis
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Assay Type
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Cell Health
- Viability
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Cell Health
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Research AreaCell Health
Features
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Device/Instrument Compatibility
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S3
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SX1
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SX5
- SX5 configured with G/R
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S3
General Specifications
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ColorGreen
Product Information
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BrandIncucyte®
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Pack Size1
One vial of 2 mg sterile lyophilized solid sufficient for 100-200 live-cell imaging phagocytosis tests in 96-well format.
4615 and 4616 are on backorder.
One cryopreserved vial of A549 cells expressing Incucyte® Nuclight Lentivirus (nuclear-labeled fluorescent protein) at a density of 1x106 cells/vial.
This product is experiencing longer than normal lead times to customers outside the United States.
One cryopreserved vial of HT-1080 cells expressing Incucyte® Nuclight Lentivirus (nuclear-labeled fluorescent protein) at a density of 1x106 cells/vial.
One cryopreserved vial of HeLa cells expressing Incucyte® Nuclight Lentivirus (nuclear-labeled fluorescent protein) at a density of 1x106 cells/vial.
One vial of lentivirus reagent (>1x106 TU/mL) suitable for transducing cells with a non-perturbing, nuclear restricted red fluorescent label driven off an EF1α promoter. Stable cell lines can be created under puromycin selection.
Top Features of Incucyte® Nuclight Red Lentivirus (EF1a, Puro)
- Label cells efficiently and homogenously using a nuclear-restricted red fluorescent protein
- Study long-term changes in proliferation by generating stably expressing cell lines
- Quantify true cell counts in mono- or co-culture kinetic assays
Popular Applications for Incucyte® Nuclight Red Lentivirus (EF1a, Puro)
- Nuclear labeling and real-time quantitation of cell proliferation and viability
- Multiplexed analyses of apoptosis and cytotoxicity assays when combined with other fluorescent reagents
- Study immune cell-killing and the interaction of immune cells with target cancer cells
One cryopreserved vial of immortalised human T lymphocyte cells stably expressing a nuclear restricted red fluorescent protein (1.5 x 106 cell/vial).
Supplied as dry powder in sufficient quantity capable of labeling between 10 and 90 million cells depending on the optimized concentration.
Top Features of Incucyte® Cytolight Rapid Dye for Live-Cell Cytoplasmic Labeling
- Rapid, efficient cytoplasmic labeling of living cells
- At optimal concentrations, non-perturbing to cell growth and morphology
- Ideal for labeling cell types that are difficult to label using BacMam, lentivirus or other transfection-based labeling approaches
Popular Applications for Incucyte® Cytolight Rapid Dye for Live-Cell Cytoplasmic Labeling
- Well-suited for identifying the location of cells within a population and monitoring the dynamic interactions between cells in mixed cultures
- Immune cell killing assays – Cytotoxic T cell killing, Antibody-dependent cell-mediated cytotoxicity (ADCC), immune cell killing in tumor spheroid models
One vial of lyophilized solid sufficient for 100-200 live-cell apoptosis tests (1 test = 1 well of 96-well microtiter plate) in 96-well format.
Top Features of Incucyte® Annexin V Dye for Apoptosis
- Detect apoptosis using a highly-selective phosphatidylserine cyanine fluorescent dye
- Track relevant biology with a dye that is non-perturbing to cell health and morphology
- Reduce loss of precious or compromised cells under-going apoptosis with mix-and-read reagents
Popular Applications for Incucyte® Annexin V Dye for Apoptosis
- Apoptosis/cell death quantification
- Immune cell killing
- Multiplexed measurements such as cytotoxicity and proliferation when combined with other Incucyte® products, including Incucyte® Caspase 3/7 Dyes, Incucyte® Nuclight Reagents, Incucyte® Cytotox Dyes and Incucyte® Confluence Metrics
Supplied as dry powder in sufficient quantity capable of labeling between 10 and 90 million cells depending on the optimized concentration.
Top Features of Incucyte® Cytolight Rapid Dye for Live-Cell Cytoplasmic Labeling
- Rapid, efficient cytoplasmic labeling of living cells
- At optimal concentrations, non-perturbing to cell growth and morphology
- Ideal for labeling cell types that are difficult to label using BacMam, lentivirus or other transfection-based labeling approaches
Popular Applications for Incucyte® Cytolight Rapid Dye for Live-Cell Cytoplasmic Labeling
- Well-suited for identifying the location of cells within a population and monitoring the dynamic interactions between cells in mixed cultures
- Immune cell killing assays – Cytotoxic T cell killing, Antibody-dependent cell-mediated cytotoxicity (ADCC), immune cell killing in tumor spheroid models