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Microsart® Calibration Reagent Mycoplasma hyorhinis

Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^8 genome copies of a specific Mycoplasma species per vial.

Item no.:  SMB95-2027

 

Microsart® Calibration Reagents provide isolated genomic DNA of a specific Mycoplasma species. Titrated genomic DNA can serve for creating standard curves by using dilutions of the material as sample in a quantitative Real-time PCR. Furthermore quantified genomic DNA can be used for specifity testing during assay validation.

To obtain the microorganisms, a special liquid culture medium was inoculated with an early passage strain and harvested at the end of the logarithmic growth phase by repeated washing and centrifugation. After DNA extraction and purification the concentration of the DNA was quantified photometrically (A 260/280) and with an ultrasensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) calibrated to weight reference standards and controlled by qPCR (compared against exactly quantified calibrator plasmids).

Catalog

Products for Microbiological Control
en 12.0 MB

Applications

  • Application
    Mycoplasma Detection

Biological Information

  • Species
    Mycoplasma hyorhinis

Components

  • Additional Required Components
    • qPCR cycler
    • PCR reaction tubes
    • centrifuge for 1.5 ml PCR tubes
    • pipettes
    • PCR kit

General Specifications

  • Detection Method
    PCR

Product Information

  • Brand
    Microsart®
  • Pack Size
    1
  • What you receive
    • 1 vial with 10^8 genome copies lyophilized
    • 3 vials buffer
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Microsart® AMP Mycoplasma
Item no.
SMB95-1001

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Microsart® Validation Standard Bacillus subtilis

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Microsart® Validation Standard Mycoplasma fermentans

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Microsart® Validation Standard are used for validating robustness and sensitivity of NAT-based detection methods, to be used for contamination control of cell cultures, cell culture media components and cell culture derived biologicals like cell autologous transplants and ATMPs.

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