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Microsart® Calibration Reagent Clostridium sporogenes

Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^8 genome copies of a specific bacterial species per vial.

Item no.:  SMB95-2033

 

Microsart® Calibration Reagents provide isolated genomic DNA of a specific bacterial species. Titrated genomic DNA can serve for creating standard curves by using dilutions of the material as sample in a quantitative Real-time PCR. Furthermore quantified genomic DNA can be used for specifity testing during assay validation.

To obtain the microorganisms, a liquid culture medium was inoculated with an early passage strain and harvested at the end of the logarithmic growth phase by repeated washing and centrifugation. After DNA extraction and purification the concentration of the DNA was quantified photometrically (A 260/280) and with an ultrasensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) calibrated to weight reference standards and controlled by qPCR (compared against exactly quantified calibrator plasmids).

Catalog

Products for Microbiological Control
en 12.0 MB

Applications

  • Application
    Bacteria Detection

Biological Information

  • Species
    Clostridium sporogenes

Components

  • Additional Required Components
    • qPCR cycler
    • PCR reaction tubes
    • centrifuge for 1.5 ml PCR tubes
    • pipettes
    • PCR kit

General Specifications

  • Detection Method
    PCR

Product Information

  • Brand
    Microsart®
  • Pack Size
    1
  • What you receive
    • 1 vial with 10^8 genome copies lyophilized
    • 3 vials buffer
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Microsart® Validation Standard Staphylococcus aureus

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Microsart® Validation Standard Spiroplasma citri

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Microsart® Validation Standard Mycoplasma fermentans

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Microsart® Validation Standard Mycoplasma gallisepticum

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Microsart® Validation Standard Mycoplasma pneumoniae

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Microsart® Validation Standard Mycoplasma orale

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Item no.
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Microsart® Validation Standards are quantified CFU preparations, containing 10 CFU per vial, optimized for validating the robustness and sensitivity of NAT-based tests.

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