Microsart® Calibration Reagent Candida glabrata
Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^6 genome copies of a specific fungal species per vial.
Item no.: SMB95-2048
Price: $410.00
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Choose your Microsart® Calibration Reagents
Microsart® Calibration Reagents provide isolated genomic DNA of a specific microbial species. Titrated genomic DNA can serve for creating standard curves by using dilutions of the material as sample in a quantitative Real-time PCR. Furthermore quantified genomic DNA can be used for specificity testing during assay validation.
To obtain the microorganisms, a liquid culture medium was inoculated with an early passage strain and harvested at the end of the logarithmic growth phase by repeated washing and centrifugation. After DNA extraction and purification the concentration of the DNA was quantified photometrically (A 260/280) and with an ultrasensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) calibrated to weight reference standards and controlled by qPCR (compared against exactly quantified calibrator plasmids).
Brochure
Catalog
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Additional Required Components
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qPCR cycler
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PCR reaction tubes
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centrifuge for 1.5 ml PCR tubes
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pipettes
- PCR kit
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qPCR cycler
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Detection MethodPCR
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SpeciesCandida glabrata
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What you receive
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1 vial with 10^6 genome copies lyophilized
- 3 vials buffer
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1 vial with 10^6 genome copies lyophilized
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ApplicationFungal Detection
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Pack Size1
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Microsart® Validation Standard are used for validating robustness and sensitivity of NAT-based detection methods, to be used for contamination control of cell cultures, cell culture media components and cell culture derived biologicals like cell autologous transplants and ATMPs.
Microsart® ATMP Extraction kit introduces a state-of-the-art (DNA-free) method for DNA extraction from cell cultures and cell culture derived biologicals, like autologous transplants and other advanced therapy medicinal products (ATMP), for subsequent DNA amplification via PCR (e.g. Microsart ATMP Bacteria and Fungi qPCR Kits).
Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^6 genome copies of a specific fungal species per vial.
Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^6 genome copies of a specific fungal species per vial.
Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^6 genome copies of a specific fungal species per vial.
Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^6 genome copies of a specific fungal species per vial.
Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^6 genome copies of a specific fungal species per vial.
Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^6 genome copies of a specific fungal species per vial.
Microsart® ATMP Sterile Release is designed for the direct detection of total bacteria and fungi in cell cultures, cell culture derived biologicals and ATMPs (e.g. autologous transplants),based on real-time PCR (qPCR).