Microsart® Calibration Reagent Candida glabrata
Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^6 genome copies of a specific fungal species per vial.
Item no.: SMB95-2048
Choose your Microsart® Calibration Reagents
Microsart® Calibration Reagents provide isolated genomic DNA of a specific microbial species. Titrated genomic DNA can serve for creating standard curves by using dilutions of the material as sample in a quantitative Real-time PCR. Furthermore quantified genomic DNA can be used for specificity testing during assay validation.
To obtain the microorganisms, a liquid culture medium was inoculated with an early passage strain and harvested at the end of the logarithmic growth phase by repeated washing and centrifugation. After DNA extraction and purification the concentration of the DNA was quantified photometrically (A 260/280) and with an ultrasensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) calibrated to weight reference standards and controlled by qPCR (compared against exactly quantified calibrator plasmids).
Applications
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ApplicationFungal Detection
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Application AreaRapid Testing
Biological Information
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SpeciesCandida glabrata
Components
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Additional Required Components
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qPCR cycler
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PCR reaction tubes
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centrifuge for 1.5 ml PCR tubes
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pipettes
- PCR kit
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qPCR cycler
Dimensions
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Filter DiameterN/A
General Specifications
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Culture MediaN/A
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Detection MethodPCR
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Membrane Color | Grid ColorN/A
Materials of Construction
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Filter MaterialN/A
Physicochemical Information
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Pore SizeN/A
Product Information
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BrandMicrosart®
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Pack SizeN/A
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TypeReagents
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What you receive
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1 vial with 10^6 genome copies lyophilized
- 3 vials buffer
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1 vial with 10^6 genome copies lyophilized
Microsart® ATMP Bacteria kit is designed for the direct detection of bacteria in cellcultures, cell culture derived biologicals and ATMPs (e.g. autologous transplants),based on real-time PCR (qPCR).
Microsart® Validation Standard are used for validating robustness and sensitivity of NAT-based detection methods, to be used for contamination control of cell cultures, cell culture media components and cell culture derived biologicals like cell autologous transplants and ATMPs.
Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^6 genome copies of a specific fungal species per vial.
Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^6 genome copies of a specific fungal species per vial.
Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^6 genome copies of a specific fungal species per vial.
Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^6 genome copies of a specific fungal species per vial.
Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^6 genome copies of a specific fungal species per vial.
Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^6 genome copies of a specific fungal species per vial.
Microsart® ATMP Sterile Release is designed to rapidly detect total bacteria and fungi in cell cultures, cell culture-derived biologicals, and ATMPs. The technology is based on real-time polymerase chain reaction (PCR).
Top Features of Microsart® ATMP Sterile Release
- Rapid detection of critical bacterial and fungal contaminants within three hours
- Designed for contamination control of ATMPs or short shelf-life applications
- The PCR kit is validated according to EP 5.1.6, EP 2.6.27, USP 1223
Popular Applications
- Lab research
- Quality control (QC)