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Microsart® Calibration Reagent Mycoplasma pneumoniae

Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^8 genome copies of a specific Mycoplasma species per vial.

Item no.:  SMB95-2024

Price:  $355.00

Availability (ex works):  For more information please log in

Microsart® Calibration Reagents provide isolated genomic DNA of a specific Mycoplasma species. Titrated genomic DNA can serve for creating standard curves by using dilutions of the material as sample in a quantitative Real-time PCR. Furthermore quantified genomic DNA can be used for specifity testing during assay validation.

To obtain the microorganisms, a special liquid culture medium was inoculated with an early passage strain and harvested at the end of the logarithmic growth phase by repeated washing and centrifugation. After DNA extraction and purification the concentration of the DNA was quantified photometrically (A 260/280) and with an ultrasensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) calibrated to weight reference standards and controlled by qPCR (compared against exactly quantified calibrator plasmids).

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Catalog

Products for Microbiological Control
en 12.0 MB

Applications

  • Application
    Mycoplasma Detection

Biological Information

  • Species
    Mycoplasma pneumoniae

Components

  • Additional Required Components
    • qPCR cycler
    • PCR reaction tubes
    • centrifuge for 1.5 ml PCR tubes
    • pipettes
    • PCR kit

Product Information

  • Pack Size
    1
  • Primary Product Type
    Kits & Assays
  • What you receive
    • 1 vial with 10^8 genome copies lyophilized
    • 3 vials buffer

Specifications

  • Detection Method
    PCR
Microsart® AMP Mycoplasma
Microsart® AMP Mycoplasma

Reduce your testing time from weeks to 3 hours with the Microsart® AMP Mycoplasma. With the ability to be very flexible in sample volume (up to 18 ml) and sample preparation, the kit is specifically designed for: the control of raw materials, for in-process control, and for lot release testing of finished products.

Item no.:   SMB95-1001

$710.00

Microsart® ATMP Mycoplasma
Microsart® ATMP Mycoplasma

The Microsart® ATMP Mycoplasma detection kit is especially designed for an easy and fast detection of mycoplasma in cell-based therapeutics like autologous chondrocyte transplants (ATMPs –advanced therapy medical products). Due to the short shelf life of these therapeutics, a fast result is needed. The PCR-technology makes it possible to reduce the time-to-result to only 3 hours.

Item no.:   SMB95-1003

$540.00

Microsart® RESEARCH Mycoplasma
Microsart® RESEARCH Mycoplasma

The Microsart® RESEARCH Mycoplasma real-time PCR kit is optimized for fast and reliable direct screening of cell cultures, cell culture supernatants and media most applicable in research and development and in-process in biopharma.

Item no.:   SMB95-1005

$335.00

Microsart® Validation Standard Mycoplasma arginini
Microsart® Validation Standard Mycoplasma arginini

Microsart® Validation Standards are quantified CFU preparations, containing 10 CFU per vial, optimized for validating the robustness and sensitivity of NAT-based tests.

Item no.:   SMB95-2011

$310.00

Microsart® Validation Standard Mycoplasma orale
Microsart® Validation Standard Mycoplasma orale

Microsart® Validation Standards are quantified CFU preparations, containing 10 CFU per vial, optimized for validating the robustness and sensitivity of NAT-based tests.

Item no.:   SMB95-2012

$310.00

Microsart® Validation Standard Mycoplasma gallisepticum
Microsart® Validation Standard Mycoplasma gallisepticum

Microsart® Validation Standards are quantified CFU preparations, containing 10 CFU per vial, optimized for validating the robustness and sensitivity of NAT-based tests.

Item no.:   SMB95-2013

$310.00

Microsart® Validation Standard Mycoplasma pneumoniae
Microsart® Validation Standard Mycoplasma pneumoniae

Microsart® Validation Standards are quantified CFU preparations, containing 10 CFU per vial, optimized for validating the robustness and sensitivity of NAT-based tests.

Item no.:   SMB95-2014

$310.00

Microsart® Validation Standard Mycoplasma synoviae
Microsart® Validation Standard Mycoplasma synoviae

Microsart® Validation Standards are quantified CFU preparations, containing 10 CFU per vial, optimized for validating the robustness and sensitivity of NAT-based tests.

Item no.:   SMB95-2015

$310.00

Microsart® Validation Standard Mycoplasma fermentans
Microsart® Validation Standard Mycoplasma fermentans

Microsart® Validation Standards are quantified CFU preparations, containing 10 CFU per vial, optimized for validating the robustness and sensitivity of NAT-based tests.

Item no.:   SMB95-2016

$310.00

Microsart® Validation Standard Mycoplasma hyorhinis
Microsart® Validation Standard Mycoplasma hyorhinis

Microsart® Validation Standards are quantified CFU preparations, containing 10 CFU per vial, optimized for validating the robustness and sensitivity of NAT-based tests.

Item no.:   SMB95-2017

$310.00

Microsart® Validation Standard Acholeplasma laidlawii
Microsart® Validation Standard Acholeplasma laidlawii

Microsart® Validation Standards are quantified CFU preparations, containing 10 CFU per vial, optimized for validating the robustness and sensitivity of NAT-based tests.

Item no.:   SMB95-2018

$310.00

Microsart® Validation Standard Spiroplasma citri
Microsart® Validation Standard Spiroplasma citri

Microsart® Validation Standards are quantified CFU preparations, containing 10 CFU per vial, optimized for validating the robustness and sensitivity of NAT-based tests.

Item no.:   SMB95-2019

$310.00

Microsart® ATMP Extraction
Microsart® ATMP Extraction

Microsart® ATMP Extraction kit introduces a state-of-the-art (DNA-free) method for DNA extraction from cell cultures and cell culture derived biologicals, like autologous transplants and other advanced therapy medicinal products (ATMP), for subsequent DNA amplification via PCR (e.g. Microsart ATMP Bacteria and Fungi qPCR Kits).

Item no.:   SMB95-2001
Microsart® Validation Standard Bacillus subtilis
Microsart® Validation Standard Bacillus subtilis

Microsart® Validation Standard are used for validating robustness and sensitivity of NAT-based detection methods, to be used for contamination control of cell cultures, cell culture media components and cell culture derived biologicals like cell autologous transplants and ATMPs.

Item no.:   SMB95-2005
Microsart® Validation Standard Pseudomonas aeruginosa
Microsart® Validation Standard Pseudomonas aeruginosa

Microsart® Validation Standard are used for validating robustness and sensitivity of NAT-based detection methods, to be used for contamination control of cell cultures, cell culture media components and cell culture derived biologicals like cell autologous transplants and ATMPs.

Item no.:   SMB95-2006
Microsart® Validation Standard Kocuria rhizophila
Microsart® Validation Standard Kocuria rhizophila

Microsart® Validation Standard are used for validating robustness and sensitivity of NAT-based detection methods, to be used for contamination control of cell cultures, cell culture media components and cell culture derived biologicals like cell autologous transplants and ATMPs.

Item no.:   SMB95-2007
Microsart® Validation Standard Clostridium sporogenes
Microsart® Validation Standard Clostridium sporogenes

Microsart® Validation Standard are used for validating robustness and sensitivity of NAT-based detection methods, to be used for contamination control of cell cultures, cell culture media components and cell culture derived biologicals like cell autologous transplants and ATMPs.

Item no.:   SMB95-2008
Microsart® Validation Standard Bacteroides vulgatus
Microsart® Validation Standard Bacteroides vulgatus

Microsart® Validation Standard are used for validating robustness and sensitivity of NAT-based detection methods, to be used for contamination control of cell cultures, cell culture media components and cell culture derived biologicals like cell autologous transplants and ATMPs.

Item no.:   SMB95-2009
Microsart® Validation Standard Staphylococcus aureus
Microsart® Validation Standard Staphylococcus aureus

Microsart® Validation Standard are used for validating robustness and sensitivity of NAT-based detection methods, to be used for contamination control of cell cultures, cell culture media components and cell culture derived biologicals like cell autologous transplants and ATMPs.

Item no.:   SMB95-2010
Microsart ATMP Sterile Release
Microsart ATMP Sterile Release

Microsart® ATMP Sterile Release is designed for the direct detection of total bacteria and fungi in cell cultures, cell culture derived biologicals and ATMPs (e.g. autologous transplants),based on real-time PCR (qPCR).

Item no.:   SMB95-1007
Microsart ATMP Bacteria (100)
Microsart ATMP Bacteria (100)

Microsart® ATMP Bacteria kit is designed for the direct detection of bacteria in cellcultures, cell culture derived biologicals and ATMPs (e.g. autologous transplants),based on real-time PCR (qPCR).

Item no.:   SMB95-1008