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Microsart® Calibration Reagent Mycoplasma orale

Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^8 genome copies of a specific Mycoplasma species per vial.

Item no.:  SMB95-2022

Price:  $355.00

Availability (ex works):  For more information please log in

   

Microsart® Calibration Reagents provide isolated genomic DNA of a specific Mycoplasma species. Titrated genomic DNA can serve for creating standard curves by using dilutions of the material as sample in a quantitative Real-time PCR. Furthermore quantified genomic DNA can be used for specifity testing during assay validation.

To obtain the microorganisms, a special liquid culture medium was inoculated with an early passage strain and harvested at the end of the logarithmic growth phase by repeated washing and centrifugation. After DNA extraction and purification the concentration of the DNA was quantified photometrically (A 260/280) and with an ultrasensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) calibrated to weight reference standards and controlled by qPCR (compared against exactly quantified calibrator plasmids).

Brochure

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Catalog

Products for Microbiological Control
en 12.0 MB

Applications

  • Application
    Mycoplasma Detection

Biological Information

  • Species
    Mycoplasma orale

Components

  • Additional Required Components
    • qPCR cycler
    • PCR reaction tubes
    • centrifuge for 1.5 ml PCR tubes
    • pipettes
    • PCR kit

Product Information

  • Pack Size
    1
  • Primary Product Type
    Kits & Assays
  • What you receive
    • 1 vial with 10^8 genome copies lyophilized
    • 3 vials buffer

Specifications

  • Detection Method
    PCR
Microsart® AMP Mycoplasma
Microsart® AMP Mycoplasma
Item no.: SMB95-1001

$710.00

Reduce your testing time from weeks to 3 hours with the Microsart® AMP Mycoplasma. With the ability to be very flexible in sample volume (up to 18 ml) and sample preparation, the kit is specifically designed for: the control of raw materials, for in-process control, and for lot release testing of finished products.

Microsart® ATMP Mycoplasma
Microsart® ATMP Mycoplasma
Item no.: SMB95-1003

$540.00

The Microsart® ATMP Mycoplasma detection kit is especially designed for an easy and fast detection of mycoplasma in cell-based therapeutics like autologous chondrocyte transplants (ATMPs –advanced therapy medical products). Due to the short shelf life of these therapeutics, a fast result is needed. The PCR-technology makes it possible to reduce the time-to-result to only 3 hours.

Microsart® RESEARCH Mycoplasma

$335.00

The Microsart® RESEARCH Mycoplasma real-time PCR kit is optimized for fast and reliable direct screening of cell cultures, cell culture supernatants and media most applicable in research and development and in-process in biopharma.

Microsart® Validation Standard Mycoplasma arginini

$310.00

Microsart® Validation Standards are quantified CFU preparations, containing 10 CFU per vial, optimized for validating the robustness and sensitivity of NAT-based tests.

Microsart® Validation Standard Mycoplasma orale

$310.00

Microsart® Validation Standards are quantified CFU preparations, containing 10 CFU per vial, optimized for validating the robustness and sensitivity of NAT-based tests.

Microsart® Validation Standard Mycoplasma gallisepticum

$310.00

Microsart® Validation Standards are quantified CFU preparations, containing 10 CFU per vial, optimized for validating the robustness and sensitivity of NAT-based tests.

Microsart® Validation Standard Mycoplasma pneumoniae

$310.00

Microsart® Validation Standards are quantified CFU preparations, containing 10 CFU per vial, optimized for validating the robustness and sensitivity of NAT-based tests.

Microsart® Validation Standard Mycoplasma synoviae

$310.00

Microsart® Validation Standards are quantified CFU preparations, containing 10 CFU per vial, optimized for validating the robustness and sensitivity of NAT-based tests.

Microsart® Validation Standard Mycoplasma fermentans

$310.00

Microsart® Validation Standards are quantified CFU preparations, containing 10 CFU per vial, optimized for validating the robustness and sensitivity of NAT-based tests.

Microsart® Validation Standard Mycoplasma hyorhinis

$310.00

Microsart® Validation Standards are quantified CFU preparations, containing 10 CFU per vial, optimized for validating the robustness and sensitivity of NAT-based tests.

Microsart® Validation Standard Acholeplasma laidlawii

$310.00

Microsart® Validation Standards are quantified CFU preparations, containing 10 CFU per vial, optimized for validating the robustness and sensitivity of NAT-based tests.

Microsart® Validation Standard Spiroplasma citri

$310.00

Microsart® Validation Standards are quantified CFU preparations, containing 10 CFU per vial, optimized for validating the robustness and sensitivity of NAT-based tests.

Microsart® ATMP Extraction
Microsart® ATMP Extraction
Item no.: SMB95-2001

Microsart® ATMP Extraction kit introduces a state-of-the-art (DNA-free) method for DNA extraction from cell cultures and cell culture derived biologicals, like autologous transplants and other advanced therapy medicinal products (ATMP), for subsequent DNA amplification via PCR (e.g. Microsart ATMP Bacteria and Fungi qPCR Kits).

Microsart® Validation Standard Bacillus subtilis

Microsart® Validation Standard are used for validating robustness and sensitivity of NAT-based detection methods, to be used for contamination control of cell cultures, cell culture media components and cell culture derived biologicals like cell autologous transplants and ATMPs.

Microsart® Validation Standard Pseudomonas aeruginosa

Microsart® Validation Standard are used for validating robustness and sensitivity of NAT-based detection methods, to be used for contamination control of cell cultures, cell culture media components and cell culture derived biologicals like cell autologous transplants and ATMPs.

Microsart® Validation Standard Kocuria rhizophila

Microsart® Validation Standard are used for validating robustness and sensitivity of NAT-based detection methods, to be used for contamination control of cell cultures, cell culture media components and cell culture derived biologicals like cell autologous transplants and ATMPs.

Microsart® Validation Standard Clostridium sporogenes

Microsart® Validation Standard are used for validating robustness and sensitivity of NAT-based detection methods, to be used for contamination control of cell cultures, cell culture media components and cell culture derived biologicals like cell autologous transplants and ATMPs.

Microsart® Validation Standard Bacteroides vulgatus

Microsart® Validation Standard are used for validating robustness and sensitivity of NAT-based detection methods, to be used for contamination control of cell cultures, cell culture media components and cell culture derived biologicals like cell autologous transplants and ATMPs.

Microsart® Validation Standard Staphylococcus aureus

Microsart® Validation Standard are used for validating robustness and sensitivity of NAT-based detection methods, to be used for contamination control of cell cultures, cell culture media components and cell culture derived biologicals like cell autologous transplants and ATMPs.

Microsart ATMP Sterile Release

Microsart® ATMP Sterile Release is designed for the direct detection of total bacteria and fungi in cell cultures, cell culture derived biologicals and ATMPs (e.g. autologous transplants),based on real-time PCR (qPCR).

Microsart ATMP Bacteria (100)
Microsart ATMP Bacteria (100)
Item no.: SMB95-1008

Microsart® ATMP Bacteria kit is designed for the direct detection of bacteria in cellcultures, cell culture derived biologicals and ATMPs (e.g. autologous transplants),based on real-time PCR (qPCR).