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Microsart® Calibration Reagents

Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^8 genome copies of a specific Mycoplasma species per vial.

 

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Products (21)

Microsart® Calibration Reagent Bacteroides vulgatus
Item no.
SMB95-2034
Brand
Microsart®
Species
Bacteroides vulgatus
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Detection Method
PCR
Microsart® Calibration Reagent Aspergillus fumigatus
Item no.
SMB95-2046
Brand
Microsart®
Species
Aspergillus fumigatus
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Detection Method
PCR
Microsart® Calibration Reagent Mycoplasma gallisepticum
Item no.
SMB95-2023
Brand
Microsart®
Species
Mycoplasma gallisepticum
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Detection Method
PCR
Microsart® Calibration Reagent Spiroplasma citri
Item no.
SMB95-2029
Brand
Microsart®
Species
Spiroplasma citri
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Detection Method
PCR
Microsart® Calibration Reagent Penicillium chrysogenum
Item no.
SMB95-2047
Brand
Microsart®
Species
Penicillium chrysogenum
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Detection Method
PCR
Microsart® Calibration Reagent Kocuria rhizophila
Item no.
SMB95-2032
Brand
Microsart®
Species
Kocuria rhizophila
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Detection Method
PCR
Microsart® Calibration Reagent Mycoplasma arginini
Item no.
SMB95-2021
Brand
Microsart®
Species
Mycoplasma arginini
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Detection Method
PCR
Microsart® Calibration Reagent Candida krusei
Item no.
SMB95-2049
Brand
Microsart®
Species
Candida krusei
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Detection Method
PCR
Microsart® Calibration Reagent Staphylococcus aureus
Item no.
SMB95-2035
Brand
Microsart®
Species
Staphylococcus aureus
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Detection Method
PCR
Microsart® Calibration Reagent Mycoplasma fermentans
Item no.
SMB95-2026
Brand
Microsart®
Species
Mycoplasma fermentans
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Detection Method
PCR
Microsart® Calibration Reagent Mycoplasma salivArium
1 Piece
Item no.
SMB95-2036
Brand
Microsart®
Species
Mycoplasma salivarium
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Detection Method
PCR

$435.00

Microsart® Calibration Reagent Mycoplasma pneumoniae
Item no.
SMB95-2024
Brand
Microsart®
Species
Mycoplasma pneumoniae
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Detection Method
PCR
Microsart® Calibration Reagent Candida albicans
Item no.
SMB95-2044
Brand
Microsart®
Species
Candida albicans
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Detection Method
PCR
Microsart® Calibration Reagent Candida glabrata
Item no.
SMB95-2048
Brand
Microsart®
Species
Candida glabrata
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Detection Method
PCR
Microsart® Calibration Reagent Candida tropicalis
Item no.
SMB95-2050
Brand
Microsart®
Species
Candida tropicalis
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Detection Method
PCR
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Microsart® Calibration Reagents provide isolated genomic DNA of a specific Mycoplasma species. Titrated genomic DNA can serve for creating standard curves by using dilutions of the material as sample in a quantitative Real-time PCR. Furthermore quantified genomic DNA can be used for specifity testing during assay validation.

To obtain the microorganisms, a special liquid culture medium was inoculated with an early passage strain and harvested at the end of the logarithmic growth phase by repeated washing and centrifugation. After DNA extraction and purification the concentration of the DNA was quantified photometrically (A 260/280) and with an ultrasensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) calibrated to weight reference standards and controlled by qPCR (compared against exactly quantified calibrator plasmids).

Catalog

Products for Microbiological Control
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