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Microsart® Calibration Reagents

Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^8 genome copies of a specific Mycoplasma species per vial.

   

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Products (20)

Microsart® Calibration Reagent Mycoplasma orale
Brand
Microsart®
Species
Mycoplasma orale
Detection Method
PCR
Microsart® Calibration Reagent Pseudomonas aeruginosa
Brand
Microsart®
Species
Pseudomonas aeruginosa
Detection Method
PCR
Microsart® Calibration Reagent Aspergillus brasiliensis
Brand
Microsart®
Species
Aspergillus brasiliensis
Detection Method
PCR
Microsart® Calibration Reagent Clostridium sporogenes
Brand
Microsart®
Species
Clostridium sporogenes
Detection Method
PCR
Microsart® Calibration Reagent Bacillus subtilis
Brand
Microsart®
Species
Bacillus subtilis
Detection Method
PCR
Microsart® Calibration Reagent Mycoplasma hyorhinis
Brand
Microsart®
Species
Mycoplasma hyorhinis
Detection Method
PCR
Microsart® Calibration Reagent Mycoplasma fermentans
Brand
Microsart®
Species
Mycoplasma fermentans
Detection Method
PCR
Microsart® Calibration Reagent Mycoplasma pneumoniae
Brand
Microsart®
Species
Mycoplasma pneumoniae
Detection Method
PCR
Microsart® Calibration Reagent Candida albicans
Brand
Microsart®
Species
Candida albicans
Detection Method
PCR
Microsart® Calibration Reagent Candida glabrata
Brand
Microsart®
Species
Candida glabrata
Detection Method
PCR
Microsart® Calibration Reagent Candida tropicalis
Brand
Microsart®
Species
Candida tropicalis
Detection Method
PCR
Microsart® Calibration Reagent Candida krusei
Brand
Microsart®
Species
Candida krusei
Detection Method
PCR
Microsart® Calibration Reagent Staphylococcus aureus
Brand
Microsart®
Species
Staphylococcus aureus
Detection Method
PCR
Microsart® Calibration Reagent Bacteroides vulgatus
Brand
Microsart®
Species
Bacteroides vulgatus
Detection Method
PCR
Microsart® Calibration Reagent Aspergillus fumigatus
Brand
Microsart®
Species
Aspergillus fumigatus
Detection Method
PCR
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Microsart® Calibration Reagents provide isolated genomic DNA of a specific Mycoplasma species. Titrated genomic DNA can serve for creating standard curves by using dilutions of the material as sample in a quantitative Real-time PCR. Furthermore quantified genomic DNA can be used for specifity testing during assay validation.

To obtain the microorganisms, a special liquid culture medium was inoculated with an early passage strain and harvested at the end of the logarithmic growth phase by repeated washing and centrifugation. After DNA extraction and purification the concentration of the DNA was quantified photometrically (A 260/280) and with an ultrasensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) calibrated to weight reference standards and controlled by qPCR (compared against exactly quantified calibrator plasmids).

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Products for Microbiological Control
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