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Microsart® Calibration Reagents

Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^8 genome copies of a specific Mycoplasma species per vial.

 

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Products (21)

Microsart® Calibration Reagent Mycoplasma orale
Item no.
SMB95-2022
Brand
Microsart®
Species
Mycoplasma orale
Application
Mycoplasma Detection
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Microsart® Calibration Reagent Mycoplasma salivArium
1 Piece
Item no.
SMB95-2036
Brand
Microsart®
Species
Mycoplasma salivarium
Application
Mycoplasma Detection
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit

$435.00

Microsart® Calibration Reagent Mycoplasma pneumoniae
Item no.
SMB95-2024
Brand
Microsart®
Species
Mycoplasma pneumoniae
Application
Mycoplasma Detection
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Microsart® Calibration Reagent Candida albicans
Item no.
SMB95-2044
Brand
Microsart®
Species
Candida albicans
Application
Fungal Detection
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Microsart® Calibration Reagent Candida glabrata
Item no.
SMB95-2048
Brand
Microsart®
Species
Candida glabrata
Application
Fungal Detection
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Microsart® Calibration Reagent Pseudomonas aeruginosa
Item no.
SMB95-2031
Brand
Microsart®
Species
Pseudomonas aeruginosa
Application
Bacteria Detection
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Microsart® Calibration Reagent Aspergillus brasiliensis
Item no.
SMB95-2045
Brand
Microsart®
Species
Aspergillus brasiliensis
Application
Fungal Detection
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Microsart® Calibration Reagent Clostridium sporogenes
Item no.
SMB95-2033
Brand
Microsart®
Species
Clostridium sporogenes
Application
Bacteria Detection
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Microsart® Calibration Reagent Bacillus subtilis
Item no.
SMB95-2030
Brand
Microsart®
Species
Bacillus subtilis
Application
Bacteria Detection
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Microsart® Calibration Reagent Mycoplasma hyorhinis
Item no.
SMB95-2027
Brand
Microsart®
Species
Mycoplasma hyorhinis
Application
Mycoplasma Detection
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Microsart® Calibration Reagent Mycoplasma fermentans
Item no.
SMB95-2026
Brand
Microsart®
Species
Mycoplasma fermentans
Application
Mycoplasma Detection
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Microsart® Calibration Reagent Bacteroides vulgatus
Item no.
SMB95-2034
Brand
Microsart®
Species
Bacteroides vulgatus
Application
Bacteria Detection
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Microsart® Calibration Reagent Aspergillus fumigatus
Item no.
SMB95-2046
Brand
Microsart®
Species
Aspergillus fumigatus
Application
Fungal Detection
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Microsart® Calibration Reagent Mycoplasma gallisepticum
Item no.
SMB95-2023
Brand
Microsart®
Species
Mycoplasma gallisepticum
Application
Mycoplasma Detection
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
Microsart® Calibration Reagent Spiroplasma citri
Item no.
SMB95-2029
Brand
Microsart®
Species
Spiroplasma citri
Application
Mycoplasma Detection
Additional Required Components
qPCR cycler, PCR reaction tubes, centrifuge for 1.5 ml PCR tubes, pipettes, PCR kit
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Microsart® Calibration Reagents provide isolated genomic DNA of a specific Mycoplasma species. Titrated genomic DNA can serve for creating standard curves by using dilutions of the material as sample in a quantitative Real-time PCR. Furthermore quantified genomic DNA can be used for specifity testing during assay validation.

To obtain the microorganisms, a special liquid culture medium was inoculated with an early passage strain and harvested at the end of the logarithmic growth phase by repeated washing and centrifugation. After DNA extraction and purification the concentration of the DNA was quantified photometrically (A 260/280) and with an ultrasensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) calibrated to weight reference standards and controlled by qPCR (compared against exactly quantified calibrator plasmids).

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Products for Microbiological Control
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