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Microsart® Calibration Reagents

Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^8 genome copies of a specific Mycoplasma species per vial.

 

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Products (8)

Microsart® Calibration Reagent Mycoplasma salivArium
1 Piece
Item no.
SMB95-2036
Application
Mycoplasma Detection
Species
Mycoplasma salivarium

$435.00

Microsart® Calibration Reagent Mycoplasma pneumoniae
Item no.
SMB95-2024
Application
Mycoplasma Detection
Species
Mycoplasma pneumoniae
Microsart® Calibration Reagent Mycoplasma hyorhinis
Item no.
SMB95-2027
Application
Mycoplasma Detection
Species
Mycoplasma hyorhinis
Microsart® Calibration Reagent Mycoplasma fermentans
Item no.
SMB95-2026
Application
Mycoplasma Detection
Species
Mycoplasma fermentans
Microsart® Calibration Reagent Mycoplasma gallisepticum
Item no.
SMB95-2023
Application
Mycoplasma Detection
Species
Mycoplasma gallisepticum
Microsart® Calibration Reagent Spiroplasma citri
Item no.
SMB95-2029
Application
Mycoplasma Detection
Species
Spiroplasma citri
Microsart® Calibration Reagent Mycoplasma arginini
Item no.
SMB95-2021
Application
Mycoplasma Detection
Species
Mycoplasma arginini
Microsart® Calibration Reagent Mycoplasma orale
Item no.
SMB95-2022
Application
Mycoplasma Detection
Species
Mycoplasma orale

Microsart® Calibration Reagents provide isolated genomic DNA of a specific Mycoplasma species. Titrated genomic DNA can serve for creating standard curves by using dilutions of the material as sample in a quantitative Real-time PCR. Furthermore quantified genomic DNA can be used for specifity testing during assay validation.

To obtain the microorganisms, a special liquid culture medium was inoculated with an early passage strain and harvested at the end of the logarithmic growth phase by repeated washing and centrifugation. After DNA extraction and purification the concentration of the DNA was quantified photometrically (A 260/280) and with an ultrasensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) calibrated to weight reference standards and controlled by qPCR (compared against exactly quantified calibrator plasmids).

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Products for Microbiological Control
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