Skip to content Skip to navigation menu

Microsart® Calibration Reagents

Microsart® Calibration Reagents are isolated genomic DNA preparations, containing 10^8 genome copies of a specific Mycoplasma species per vial.

 

Choose your product

Products (6)

Microsart® Calibration Reagent Pseudomonas aeruginosa
Item no.
SMB95-2031
Application
Bacteria Detection
Species
Pseudomonas aeruginosa
Microsart® Calibration Reagent Clostridium sporogenes
Item no.
SMB95-2033
Application
Bacteria Detection
Species
Clostridium sporogenes
Microsart® Calibration Reagent Bacillus subtilis
Item no.
SMB95-2030
Application
Bacteria Detection
Species
Bacillus subtilis
Microsart® Calibration Reagent Bacteroides vulgatus
Item no.
SMB95-2034
Application
Bacteria Detection
Species
Bacteroides vulgatus
Microsart® Calibration Reagent Kocuria rhizophila
Item no.
SMB95-2032
Application
Bacteria Detection
Species
Kocuria rhizophila
Microsart® Calibration Reagent Staphylococcus aureus
Item no.
SMB95-2035
Application
Bacteria Detection
Species
Staphylococcus aureus

Microsart® Calibration Reagents provide isolated genomic DNA of a specific Mycoplasma species. Titrated genomic DNA can serve for creating standard curves by using dilutions of the material as sample in a quantitative Real-time PCR. Furthermore quantified genomic DNA can be used for specifity testing during assay validation.

To obtain the microorganisms, a special liquid culture medium was inoculated with an early passage strain and harvested at the end of the logarithmic growth phase by repeated washing and centrifugation. After DNA extraction and purification the concentration of the DNA was quantified photometrically (A 260/280) and with an ultrasensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) calibrated to weight reference standards and controlled by qPCR (compared against exactly quantified calibrator plasmids).

Catalog

Products for Microbiological Control
en 12.0 MB