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iQue® Human T Cell Exhaustion Kit

The Human T Cell Exhaustion Cell and Cytokine Profiling Kit is designed for ease of use in multiplexing markers of T cell exhaustion, phenotyping T helper and T cytotoxic cells, and bead-based measurement of secreted cytokines, all in the same assay. This optimized assay offers these unique advantages:

  • Live immune cells are distinguished from dead or membrane compromised cells by staining with a fluorescent membrane integrity dye.

  • Cells are immunophenotyped by staining with a fluorescent antibody panel to distinguish CD3+ T cells, CD3- non-T cells, CD3+CD4+ helper T cells, and CD3+CD8+ cytotoxic T cells.

  • The panel also includes three markers of T cell exhaustion: PD-1, Tim-3, and Lag-3.

  • Optimized workflow enables the measurement of cytokines secreted by activated T cells (IFNγ and TNF) in the same assay well.

  • The capacity of the sample cells to proliferate can also be determined with an optional proliferation dye if desired (included with the kit).

Item no.:  97069

   

Key Benefits

Speed: Sample to actionable data by allowing multiple assays to be run in one assay thereby reducing the hands-on time, numbers of overall tests and conserving precious samples.

Results: 11 endpoints from a single-well assay measuring T cell exhaustion phenotypes and function, as well as cytokine secretion in response to stimulation, cell viability, and an optional proliferation dye allows screening for molecules that reverse T cell exhaustion.

Biological insight: Generate functional data and analyze cell health from the same well by multiplexing cell and cytokine detection in one assay.

Simplicity: Single platform assay, data acquisition, and analysis eliminate the time of acquiring and correlating data from multiple sources.

Biological Information

  • Target
    Human

General Specifications

  • Laser Configuration
    Violet-Blue-Red
  • Reagents and Kits Type
    T Cell Exhaustion

eShop Dynamic Attributes

  • Application
    Immune Cell Function and Characterization
  • Pack Size
    1 x 96
  • Research Area
    • Immuno-Oncology
    • Antibody Discovery
    • Drug Discovery