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Sartobind® D A4 Sheet

Sartobind IEX membranes are macroporous, cellulose-based membranes carrying IEX ligands. Binding of charged target molecules takes place at high flow rates.

Order Number:  94IEXD42-001

Availability:  On Request

Sartobind ion exchange (IEX) membranes feature binding sites that are grafted homogenously as an approximately 0.5 - 1 µm film on the inner walls of the reinforced and cross-linked cellulose network. Diffusion time in adsorbers is negligible because of the large pores and immediate binding of the target substance to the ligands. There is no pore diffusion as given in conventional beads but film diffusion on any point of the microporous membrane structure. At convective flow conditions the movement of the molecules of the mobile phase is directed by pump pressure only. That is why membrane adsorbers feature extremely short cycle times and exceptionally high flow rate and throughput..

  • Macroporous
  • High flow rate
  • Simple handling

Brochure

Sartobind® Membrane Adsorbers for Rapid Purification of Proteins
en 1.3 MB

User Manual

Sartobind® Ion Exchange Sheet — A Separation Technology Based on Microporous Membranes
en 138.3 kB

Product properties

  • Membrane material
    Stabilised reinforced cellulose
  • Dimensions
    21 cm × 29.7 cm
  • Membrane area
    624 cm²
  • Pore size
    3 - 5 µm
  • Typical dynamic binding capacity 10 %
    >29 mg/mL, >0.8 mg/cm²
  • Pack size
    1

Technical attributes

  • What you receive
    1 sheet` 1 manual
  • Reinforcement material
    Polyester
  • Ligand
    Diethylamine
  • Thermal stability
    autoclavable at 121°C
  • Ligand density
    4 – 6 µeq/cm²
  • Membrane thickness
    275 µm
  • Adsorption area
    624 cm²
  • Flow rate at 1 bar
    > 80 mL/cm² × min
  • Chemical compatibility
    Stable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents
  • Reference protein
    Bovine serum albumin
  • Membrane type
    Weak anion exchanger
  • pH stability
    pH 2 – 14 (short term), pH 4 – 13 (long term)
  • Reusable
    Yes