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Vivaflow 200, 30,000 MWCO Hydrosart

0.5 - 5 l

  • Plug and play laboratory cross flow cassette
  • Re-usable
  • Optimal for cell culture supernatant and virus concentration
  • High flow rate and recovery

Order Number:  VF20H2

Availability:  On Request

Vivaflow® 200 is a ready to use and reusable laboratory crossflow cassette for concentrating up to 5 l samples. The membrane surface per device is 200 cm². One unit comes with all necessary accessories for running the device with a laboratory pump and a size 16 pump head. For speeding up the concentration, two cassettes can be run simultaneously.

Vivaflow® 200 is optimally suited for applications like cell culture supernatant concentration, virus concentration and water concentration e.g. in fish breeding facilities. This economical product offers a standard of ease of use, reliability and flexibility unrivalled by any laboratory filtration system in the market. The thin-channel, flip-flow recirculation path of the modules provides high crossflow velocities with minimum pump speed requirements. A single 200 cm2 module typically concentrates 1 l by a factor of 50 in less than 30 min. Alternatively, two Vivaflow® 200 modules can be used in parallel for concentration of 5 l in under 75 minutes. Near total recovery of the concentrate is achieved with a single final rinse. Vivaflow® 200 can be easily cleaned and re-used several times. All accessories for sample concentration, except a peristaltic pump with a pump head for size 16 tubing, are included in the package for using a single cassette. A 500 ml sealed diafiltration reservoir can be added to the system for rapid and convenient buffer exchange.

Equipment required

The standard Vivaflow® 200 package comes with tubing, pressure indicator, flow restrictor and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16).

Equipment required

The standard Vivaflow® 200 package comes with tubing, pressure indicator, flow restrictor and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16).

Running 2 Vivaflow® 200 cassettes in parallel

For speeding up the concentration, two Vivaflow® 200 cassettes can easily be connected using a Y connector which is preassembeled with the correct tubing sizes and fittings (size 16 tubing for connecting the two Vivaflow® 200 units, size 15 tubing for the pump). Please note that you need a size 15 pump head for running 2 Vivaflows® in parallel.

  • High flow rate and recovery
  • Clear housing for process monitoring
  • Ready to use single cassettes
  • Easy set up of 2 cassettes for use in parallel
  • Re-usable product

Performance Characteristics

  Time to concentrate up to 20x (min.) at 3 bar
inlet pressure, 20°C
 Start volumeAverage fluxRecovery % 
 1 litreml/minDirect25 ml rinse
Lysozyme 0.25 mg/ml (14,000 MW)
2,000 MWCO Hydrosart160697 %>99 %
3,000 MWCO PES180597 %>99 %
BSA 1.0 mg/ml (66,000 MW)
5,000 MWCO PES293398 %>99 %
5,000 MWCO Hydrosart701498 %>99 %
10,000 MWCO PES234196 %>99 %
10,000 MWCO Hydrosart352798 %>99 %
30,000 MWCO PES253896 %99 %
30,000 MWCO Hydrosart204896 %>99 %
50,000 MWCO PES224396 %98 %
γ Globulins 1.0 mg/ml (average 160,000 MW)
100,000 MWCO PES541896 %99 %
100,000 MWCO RC452196 %99 %
Yeast 1.0 mg/ml (S. Cerevisiae)
0.2 μm PES118692 %98 %
Dilute solute concentration, start volume 1 litre at 3 bar, 10,000 MWCO PES
BSA 0.001 mg/ml185290 %98 %
BSA 0.01 mg/ml204792 %98 %
BSA 0.1 mg/ml214594 %99 %
Start volume 5 litres (two VF200 in parallel at 3 bar) 10,000 MWCO PES
BSA 1.0 mg/ml (66,000 MW)677097 %>99 %

Application Note

Sartorius Ultrafiltration Products in the Preparation of Biological and Medical Nanocarriers – a Short Review
en 133.1 kB
Vivaflow® and Vivaspin® Workflow in Protein Research Laboratories
en 827.9 kB
The Measurement of Soluble (< 10 kDa) Trace Metals in seawater by the Vivaflow 50
en 269.6 kB

Brochure

Ultrafiltration Family
en 1.6 MB

Catalog

Laboratory Filtration Products (US and Canada)
en 9.6 MB
Laboratory Filtration Products
en 14.3 MB

Datasheet

Vivaflow® 50 | 50R | 200 — Unique, “Plug & Play” Laboratory Crossflow Devices
en 677.1 kB

User Manual

Vivaflow 50 | 50R | 200
en 731.4 kB
Vivaflow 50|200
fr 543.1 kB

Product properties

  • MWCO
    30,000 Daltons
  • Width
    38 mm
  • Volume Range
    500 – 5,000 mL
  • Max. operating pressure
    4 bar
  • Primary Product Type
    Ultrafiltration Devices
  • Membrane material
    Hydrosart®
  • Membrane
    Hydrosart®
  • Tubing
    PVC (medical grade)
  • Material o-ring | seal
    Silicone (SI)

Technical attributes

  • Electrical / battery item
    false
  • Housing material
    Acrylic
  • Pressure indicator
    Polypropylene, SS spring
  • Flow Channel
    Acrylic
  • Flow Restrictor
    Polypropylene (PP)
  • Maximum Pressure
    4 bar
  • Overall length
    127 mm
  • Hold-up volume
    5.3 mL
  • Active Membrane Area
    200 cm²
  • Overall height
    138 mm
  • Max. temperature
    60° C
  • Non-recoverable Hold-up
    < 2 mL
  • Channel width
    10 mm
  • Minimum Recirculation Volume
    < 20 mL
  • Channel height
    0.4 mm
  • Pump Flow
    200 – 400 mL/min
  • Certification
    None
  • Application
    Protein Concentration
  • Area of application
    Buffer Exchange` Protein/DNA Concentration
  • Market
    BioPharma` Research
  • Device type
    Tangential / Crossflow Concentrator
  • Sample Type
    Protein` Nanoparticles` Virus
  • Fittings
    Nylon (NY)
  • Membrane support
    Polypropylene (PP)