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UltraCULTURE™ serum-free medium consists of a DMEM:F-12 base which is supplemented with recombinant human insulin, bovine transferrin and a purified mixture of bovine serum proteins including albumin. The total protein concentration of UltraCULTURE™ Medium is approximately 3 mg/mL.
UltraCULTURE™ Medium does not contain L-glutamine. UltraCULTURE™ Medium has been used to grow a variety of cells of primary origin and established cell lines. The medium may be used to support the fusion of cells during the formation of hybridomas.
UltraCULTURE™ Medium has been used to grow cells of lymphoid origin including monocyte and macrophage cell lines. Epithelial and fibroblastic cells have been cultivated in UltraCULTURE™ Medium and endothelial cells have been cultivated under reduced serum concentration (5% fetal bovine serum using UltraCULTURE™ Medium as a base formulation).
UltraCULTURE™ Medium has also been used as a complete medium to support the generation of viral particles for use in vaccine production. UltraCULTURE™ Medium may be supplemented with cryoprotective medium to cryopreserve cells in a serum-free environment.