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Insect-XPRESS™ Medium

Insect-XPRESS is a popular protein-free medium designed for use on invertebrate cell lines including Sf9, Sf21, and Hi-5

Insect-XPRESS™ Protein-free Insect Cell Medium is a formulation designed to support the growth of insect cell lines derived from Spodoptera frugiperda (Sf9 and Sf21). Cell densities in excess of 8.3 × 106

cells/ml can be achieved with suspension cultures of Sf9 cells using Insect-XPRESS Medium and an excess of oxygen.

   

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Products (1)

INSECT-XPRESS W/ L-GLN 1 L
INSECT-XPRESS W/ L-GLN 1 L
Item no.: BEBP12-730Q
Adherence/Suspension
Adherent/Suspension
Animal Origin (AO) / Non-Animal Origin (NAO)
AO
Packaging
1 L
Delivery Condition
Sterile
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Insect-XPRESS Medium was developed to support the growth of invertebrate cell lines, derived from the fall army worm, Spodoptera frugiperda mainly Sf9 and Sf21. The Insect-XPRESS Medium formulation is protein-free (all components are less than or equal to 10,000 mwt.) and has been designed to support the growth of insect cells under attachmentdependent or attachment-independent conditions.

Cell densities in excess of 8.3 + 106 cells/mL have been achieved with suspension cultures of Sf9 cells using Insect-XPRESS Medium and an excess of oxygen. Insect-XPRESS Medium may also be used for stationary monolayer cultures and shakeflask cultures. The Insect-XPRESS 1X liquid formulation contains L-glutamine and supports the production of recombinant proteins by cells infected with viral vectors such as the Baculovirus. Insect-XPRESS Medium may be used as a base for the cryopreservation of insect cells. We recommend that the Insect-XPRESS Medium liquid be mixed 50:50 with Cryoprotective Medium prior to cryopreservation.

Serum-free media and reagents have a wide range of applications, including production of monoclonal antibodies, viral antigens, and recombinant proteins using a variety of mammalian and invertebrate cell lines. There are numerous advantages associated with the use of serum-free media formulations.

  • No need for serum qualification
  • Increased definition
  • Simplified purification and downstream processing
  • Better control over the physiological condition of cultures
  • Ability to optimize formulations for specific cell types